Abstract
Background: The p53 tumor suppressor gene is altered in up to 70% of colorectal cancers. Materials and Methods: We infected the colorectal cancer cell lines SW620 and KM12L4, in which p53 is mutated, with the replication-defective adenovirus Ad5/CMV/p53 to evaluate the effects of adenovirus-mediated wild-type p53 gene transfer Gene transduction was measured by cytochemical staining of cells infected with the Ad5/CMV/β-gal virus and expression of the wildtype p53 protein in these cells was demonstrated by immunoblotting. Results: Significant suppression of in vitro cell proliferation and induction of apoptosis (as measured by TUNEL assay labeling) were observed following Ad5/CMV/p53 infection. More importantly, similar effects were observed in vivo in an established nude mouse subcutaneous tumor model; significant suppression of tumor growth (60% 70%) and induction of apoptosis were observed following intratumoral injections of Ad5/CMV/p53. Conclusion: This form of therapy may provide a novel approach to colorectal cancer.
Original language | English (US) |
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Pages (from-to) | 3415-3422 |
Number of pages | 8 |
Journal | Anticancer research |
Volume | 16 |
Issue number | 6 B |
State | Published - 1996 |
Keywords
- Adenovirus
- Colon cancer
- Gene therapy
- p53
ASJC Scopus subject areas
- Oncology
- Cancer Research