In vivo and in vitro effects of cigarette smoke condensate fractions

Smoke condensate derived from the low nicotine reference cigarette, IAI, was fractioned for use by standard procedures. Fractions BI(a), BI(b), N(MeOH) and N(NM) were potent inducers, fractions B(e), WA(I), WA(e), N(CH) as well as the starting material (SM) and reconstituted fractions (RF) were weaker inducers, and B(W), SA(I), SA(W), SA(e) had no effect or inhibited pulmonary aryl hydrocarbon hydroxylase (AHH) activity. Simultaneous treatment with fractions BI(b), SA(e), and N(MeOH) markedly enhanced (>35%) the incidence of tumors in C3H/f Mai mice treated subcutaneously with 10μg 3 methylcholanthrene, while fractions BI(a), WA(I) and SA(I) also enhanced susceptibility, but to a lesser extent (>25%). The SM, RF and fractions BI(b) and WA(I) malignantly transformed the 10T1/2 CL8 cells. Fractions BI(a), BI(b), and WA(I) contained about 80% of the total mutagenic activity of the starting or reconstituted condensate. Thus, at least two fractions, the BI(b) and WA(I) contain chemicals that can induce AHH activity and, most likely, certain metabolites generated by the action of this enzyme are mutagenic, oncogenic in tissue culture, and co carcinogenic in vivo.