In vivo evaluation of 2′-deoxy-2′-[18F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([18F]FIAU) and 2′-deoxy-2′-[18F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression

Mian M. Alauddin; Antranic Shahinian; Ryan Park; Michel Tohme; John D. Fissekis; Peter S. Conti

doi:10.1007/s00259-006-0305-1

In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression

Mian M. Alauddin, Antranic Shahinian, Ryan Park, Michel Tohme, John D. Fissekis, Peter S. Conti

Cancer Systems Imaging

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Purpose: FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods: In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [¹⁴C]FIAU and [³H]FEAU. PET imaging was performed using [¹⁸F]FIAU and [¹⁸F]FEAU. Results: In vitro uptake of [¹⁴C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p < 0.001) than in wild type cells at 30, 60, and 120 min, respectively. Uptake of [ ³H]FEAU in transduced cells was 46-fold, 62-fold, and 121-fold higher (p < 0.001) than in wild type cells at the same time points. In vivo uptake of [¹⁴C]FIAU at 2 h in HSV1-tk positive tumors was 15.48 ± 3.94, 6.7-fold higher (p < 0.001) than in wild type tumors. Uptake of [ ³H]FEAU in transduced tumors was 9.98 ± 1.99, 5.0-fold higher (p < 0.001) than in wild type tumors. Micro-PET images using [ ¹⁸F]FIAU and [¹⁸F]FEAU also showed very high uptake in HSV-tk tumors. Conclusion: [¹⁸F]FIAU and [¹⁸F]FEAU appear to be potential PET imaging agents for gene expression.

Original language	English (US)
Pages (from-to)	822-829
Number of pages	8
Journal	European Journal of Nuclear Medicine and Molecular Imaging
Volume	34
Issue number	6
DOIs	https://doi.org/10.1007/s00259-006-0305-1
State	Published - Jun 2007

Keywords

FEAU
FIAU
Gene expression
HSV-tk
PET

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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10.1007/s00259-006-0305-1

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Alauddin, M. M., Shahinian, A., Park, R., Tohme, M., Fissekis, J. D., & Conti, P. S. (2007). In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression. European Journal of Nuclear Medicine and Molecular Imaging, 34(6), 822-829. https://doi.org/10.1007/s00259-006-0305-1

In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression. / Alauddin, Mian M.; Shahinian, Antranic; Park, Ryan et al.
In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 34, No. 6, 06.2007, p. 822-829.

Research output: Contribution to journal › Article › peer-review

Alauddin, MM, Shahinian, A, Park, R, Tohme, M, Fissekis, JD & Conti, PS 2007, 'In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression', European Journal of Nuclear Medicine and Molecular Imaging, vol. 34, no. 6, pp. 822-829. https://doi.org/10.1007/s00259-006-0305-1

Alauddin MM, Shahinian A, Park R, Tohme M, Fissekis JD, Conti PS. In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression. European Journal of Nuclear Medicine and Molecular Imaging. 2007 Jun;34(6):822-829. doi: 10.1007/s00259-006-0305-1

Alauddin, Mian M. ; Shahinian, Antranic ; Park, Ryan et al. / In vivo evaluation of 2′-deoxy-2′-[¹⁸F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([¹⁸F]FIAU) and 2′-deoxy-2′-[¹⁸F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([¹⁸F]FEAU) as markers for suicide gene expression. In: European Journal of Nuclear Medicine and Molecular Imaging. 2007 ; Vol. 34, No. 6. pp. 822-829.

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title = "In vivo evaluation of 2′-deoxy-2′-[18F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([18F]FIAU) and 2′-deoxy-2′-[18F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression",

abstract = "Purpose: FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods: In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. Results: In vitro uptake of [14C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p < 0.001) than in wild type cells at 30, 60, and 120 min, respectively. Uptake of [ 3H]FEAU in transduced cells was 46-fold, 62-fold, and 121-fold higher (p < 0.001) than in wild type cells at the same time points. In vivo uptake of [14C]FIAU at 2 h in HSV1-tk positive tumors was 15.48 ± 3.94, 6.7-fold higher (p < 0.001) than in wild type tumors. Uptake of [ 3H]FEAU in transduced tumors was 9.98 ± 1.99, 5.0-fold higher (p < 0.001) than in wild type tumors. Micro-PET images using [ 18F]FIAU and [18F]FEAU also showed very high uptake in HSV-tk tumors. Conclusion: [18F]FIAU and [18F]FEAU appear to be potential PET imaging agents for gene expression.",

keywords = "FEAU, FIAU, Gene expression, HSV-tk, PET",

author = "Alauddin, {Mian M.} and Antranic Shahinian and Ryan Park and Michel Tohme and Fissekis, {John D.} and Conti, {Peter S.}",

note = "Funding Information: Acknowledgement This work was supported in part by National Cancer Institute Grants CA 72896 and P20 CA86532.",

year = "2007",

month = jun,

doi = "10.1007/s00259-006-0305-1",

language = "English (US)",

volume = "34",

pages = "822--829",

journal = "European Journal of Nuclear Medicine and Molecular Imaging",

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T1 - In vivo evaluation of 2′-deoxy-2′-[18F]fluoro-5- iodo-1-β-D-arabinofuranosyluracil ([18F]FIAU) and 2′-deoxy-2′-[18F]fluoro-5-ethyl-1-β-D- arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression

AU - Alauddin, Mian M.

AU - Shahinian, Antranic

AU - Park, Ryan

AU - Tohme, Michel

AU - Fissekis, John D.

AU - Conti, Peter S.

N1 - Funding Information: Acknowledgement This work was supported in part by National Cancer Institute Grants CA 72896 and P20 CA86532.

PY - 2007/6

Y1 - 2007/6

N2 - Purpose: FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods: In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. Results: In vitro uptake of [14C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p < 0.001) than in wild type cells at 30, 60, and 120 min, respectively. Uptake of [ 3H]FEAU in transduced cells was 46-fold, 62-fold, and 121-fold higher (p < 0.001) than in wild type cells at the same time points. In vivo uptake of [14C]FIAU at 2 h in HSV1-tk positive tumors was 15.48 ± 3.94, 6.7-fold higher (p < 0.001) than in wild type tumors. Uptake of [ 3H]FEAU in transduced tumors was 9.98 ± 1.99, 5.0-fold higher (p < 0.001) than in wild type tumors. Micro-PET images using [ 18F]FIAU and [18F]FEAU also showed very high uptake in HSV-tk tumors. Conclusion: [18F]FIAU and [18F]FEAU appear to be potential PET imaging agents for gene expression.

AB - Purpose: FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods: In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. Results: In vitro uptake of [14C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p < 0.001) than in wild type cells at 30, 60, and 120 min, respectively. Uptake of [ 3H]FEAU in transduced cells was 46-fold, 62-fold, and 121-fold higher (p < 0.001) than in wild type cells at the same time points. In vivo uptake of [14C]FIAU at 2 h in HSV1-tk positive tumors was 15.48 ± 3.94, 6.7-fold higher (p < 0.001) than in wild type tumors. Uptake of [ 3H]FEAU in transduced tumors was 9.98 ± 1.99, 5.0-fold higher (p < 0.001) than in wild type tumors. Micro-PET images using [ 18F]FIAU and [18F]FEAU also showed very high uptake in HSV-tk tumors. Conclusion: [18F]FIAU and [18F]FEAU appear to be potential PET imaging agents for gene expression.

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