In vivo 19F-magnetic resonance imaging of adoptively transferred NK cells

Srinivas S. Somanchi, Bridget A. Kennis, Vidya Gopalakrishnan, Dean A Lee, James A. Bankson

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Scopus citations

Abstract

In order to assess the biodistribution, homing, and persistence of adoptively transferred natural killer (NK) cell immunotherapies, there is a need for imaging methodology suitable for use in preclinical studies with relevance to clinical translation. Amongst the available approaches, 19F-MRI is very appealing for in vivo imaging due to the absence of background signal, enabling clear detection of 19F labeled cells in vivo. Here we describe a methodology for in vivo imaging of adoptively transferred NK cells labeled with 19F nanoemulsion, using clinically translatable technology of 19F/1H magnetic resonance imaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages317-332
Number of pages16
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1441
ISSN (Print)1064-3745

Keywords

  • Adoptive immunotherapy
  • F
  • In vivo imaging
  • Magnetic resonance imaging
  • NK cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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