Abstract
Dendritic cells (DC) serve as the professional antigen presenting cells for initiating strong antigen-specific cellular immune responses. We reported earlier protection of rhesus macaques from chronic infection and AIDS by immunizing with DC pulsed ex vivo with a conserved HIV envelope peptide-cocktail vaccine for efficient priming of cellular immune responses. In the present study, we attempted to target DC in vivo by combining subcutaneous administration of fins-like tyrosine 3 kinase ligand (FL) and immunization with the envelope peptide-cocktail and synthetic oligodeoxynucleotide containing unmethylated CpG motifs as adjuvant. The vaccinated animals exhibited transient mobilization of DC into peripheral blood and priming of T cells with antigen specific proliferation, IFN-γ production as well as cytolytic activity. Polychromatic flow cytometry analyses revealed the presence of antigen-specific CD4 memory T cells producing cytokines in the vaccinated animals, but not the mock-vaccinated controls. Pathogenic SHIV challenge provided marginal protective efficacy suggesting the need for further improvements to realize the full potential of the in vivo DC-targeting strategy for delivering HIV candidate vaccines.
Original language | English (US) |
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Pages (from-to) | 37-48 |
Number of pages | 12 |
Journal | Current Topics in Peptide and Protein Research |
Volume | 11 |
State | Published - 2010 |
Keywords
- Cell-mediated immunity
- Dendritic cells
- Flt-3 ligand
- Peptide vaccine
- Rhesus macaques
- SHIV
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology