Abstract
The purpose of these studies was to demonstrate causal effects of abnormalities induced in mouse chromosome 14 on tumorigenicity and metastasis using the K-1735 murine melanoma cell line. Because anomalies in chromosome 14 have previously been associated with increases in metastatic potential, we induced chromosome 14 anomalies in a nonmetastatic K-1735 clone 10 cells initially containing two normal copies of chromosome 14 by treatment with mitomycin C. Clone 10-M1, in which a small population of cells (approximately 4%) contained translocations involving chromosome 14, was isolated and injected into athymic nude mice. Unlike the parental C-10 cells, C-10 M1 cells produced experimental lung metastases. Chromosomal analysis of cell cultures established from both subcutaneous tumors and lung metastases indicated that approximately 35% of the cell population contained chromosome 14 anomalies suggesting that this chromosome may play a role in tumor growth and metastasis.
Original language | English (US) |
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Pages (from-to) | 119-125 |
Number of pages | 7 |
Journal | Cancer Genetics and Cytogenetics |
Volume | 98 |
Issue number | 2 |
DOIs | |
State | Published - Oct 15 1997 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research