Abstract
2-methoxyestradiol (2-MeOE2) treatment caused significant growth inhibition of H460 and A549 human lung cancer cell lines which contain wild-type p53. However, 2-MeOE2 had a little effect on the p53 negative H358 and p53 mutated H322 cell lines. Western blot analysis indicated that 2-MeOE2 treatment resulted in an eightfold increase in the endogenous wild-type p53 protein, while the level of the mutant p53 protein remained unchanged. TdT staining indicated that following ZMeOE2-mediated increases in wildtype p53 protein, cells bypass the G1-S checkpoint of the cell cycle with 30 to 40% undergoing apoptosis. Introduction of anti-sense wt-p53 into wt-p53 cells abrogated the 2-MeOE2 effect. A significant portion of lung cancer retains the wild-type p53 gene therefore, 2-MeOE2 may have therapeutic application.
Original language | English (US) |
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Pages (from-to) | 379-384 |
Number of pages | 6 |
Journal | Oncogene |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - 1997 |
Keywords
- Apoptosis
- Gene expression
- Lung cancer
- Methoxyestradiol
- p53
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research