TY - JOUR
T1 - Induction of N-Acetylmannosamine catabolic pathway in yeast
AU - Biswas, Manika
AU - Singh, Balraj
AU - Datta, Asis
N1 - Funding Information:
The work was supportedin part by a grant \[No. 23-461/76( SR II)\] from the UniversityG rantsC ommissionN, ew Delhi, India. M. B. is a predoctoral fellow supportebdy Kothari Scientifica nd ResearchIn stituteC, alcuttaI,n dia.
PY - 1979/7/18
Y1 - 1979/7/18
N2 - N-Acetylmannosamine kinase activity is absent from yeast cells grown on N-acetylmannosamine. However, other enzymes of the catabolic pathway, namely, N-acetylmannosamine-2-epimerase, N-acetylglucosamine kinase and glucosamine-6-phosphate deaminase are induced. In addition, a high affinity uptake system (permease) for the uptake of N-acetylglucosamine is synthesized under these conditions. The presence of either N-acetylmannosamine or N-acetylglucosamine as inducer is essential for the induced synthesis of these enzymes. The enzyme synthesis stops and their concentration in the cells declines rapidly as soon as inducer is removed from the medium. N-Acetyl-D-galactosamine can also induce all these enzymes except for N-acetylmannosamine-2-epimerase, suggesting the convergence of catabolic pathways for both the aminosugars at N-acetyl-D-glucosamine. Experiments with inhibitors of macromolecule synthesis suggest that the synthesis of RNA and protein is necessary for the induction of these enzymes whereas the synthesis of DNA is not.
AB - N-Acetylmannosamine kinase activity is absent from yeast cells grown on N-acetylmannosamine. However, other enzymes of the catabolic pathway, namely, N-acetylmannosamine-2-epimerase, N-acetylglucosamine kinase and glucosamine-6-phosphate deaminase are induced. In addition, a high affinity uptake system (permease) for the uptake of N-acetylglucosamine is synthesized under these conditions. The presence of either N-acetylmannosamine or N-acetylglucosamine as inducer is essential for the induced synthesis of these enzymes. The enzyme synthesis stops and their concentration in the cells declines rapidly as soon as inducer is removed from the medium. N-Acetyl-D-galactosamine can also induce all these enzymes except for N-acetylmannosamine-2-epimerase, suggesting the convergence of catabolic pathways for both the aminosugars at N-acetyl-D-glucosamine. Experiments with inhibitors of macromolecule synthesis suggest that the synthesis of RNA and protein is necessary for the induction of these enzymes whereas the synthesis of DNA is not.
KW - (Yeast)
KW - Aminosugar metabolism
KW - N-Acetylmannosamine 2-epimerase synthesis
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U2 - 10.1016/0304-4165(79)90186-7
DO - 10.1016/0304-4165(79)90186-7
M3 - Article
C2 - 223651
AN - SCOPUS:0018795077
SN - 0304-4165
VL - 585
SP - 535
EP - 542
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 4
ER -