Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells

Tatyana N. Lepikhova; Elena V. Navolotskaya; Tatyana A. Zargarova; Rosa I. Nurieva; Valery M. Lipkin; Vladimir P. Zav'yalov

doi:10.1016/S0196-9781(00)00152-2

Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells

Tatyana N. Lepikhova, Elena V. Navolotskaya, Tatyana A. Zargarova, Rosa I. Nurieva, Valery M. Lipkin, Vladimir P. Zav'yalov

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Influence of the ACTH-like peptide H-Val-Lys-Lys-Pro-Gly-Ser-Ser-Val- Lys-Val-OH corresponding to the sequence 11-20 of the variable part of human immunoglobulin G1 (IgG1) heavy chain on growth of MT-4 human T-lymphoblastoid cell line was investigated. It was found that the ACTH-like peptide at concentration range 10^-11 -10^-7 M inhibits the proliferation of MT-4 cells. Labeled ACTH 'address segment' [¹²⁵I]ACTH (13-24) was used to establish that MT-4 cells express specific receptors for ACTH (K(d) = 97 pM). The ACTH-like peptide and human ACTH (but not IgG1 heavy chain) were shown to compete with [¹²⁵I]ACTH (13-24) for binding to these receptors (K(i1) = 0.38 nM and K(i2) = 0.34 nM). (C) 2000 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	353-357
Number of pages	5
Journal	Peptides
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(00)00152-2
State	Published - Mar 2000
Externally published	Yes

Keywords

ACTH (Adrenocorticotropin)
Growth inhibition
MT-4 cells
Peptide
Receptor

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(00)00152-2

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title = "Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells",

abstract = "Influence of the ACTH-like peptide H-Val-Lys-Lys-Pro-Gly-Ser-Ser-Val- Lys-Val-OH corresponding to the sequence 11-20 of the variable part of human immunoglobulin G1 (IgG1) heavy chain on growth of MT-4 human T-lymphoblastoid cell line was investigated. It was found that the ACTH-like peptide at concentration range 10-11 -10-7 M inhibits the proliferation of MT-4 cells. Labeled ACTH 'address segment' [125I]ACTH (13-24) was used to establish that MT-4 cells express specific receptors for ACTH (K(d) = 97 pM). The ACTH-like peptide and human ACTH (but not IgG1 heavy chain) were shown to compete with [125I]ACTH (13-24) for binding to these receptors (K(i1) = 0.38 nM and K(i2) = 0.34 nM). (C) 2000 Elsevier Science Inc.",

keywords = "ACTH (Adrenocorticotropin), Growth inhibition, MT-4 cells, Peptide, Receptor",

author = "Lepikhova, {Tatyana N.} and Navolotskaya, {Elena V.} and Zargarova, {Tatyana A.} and Nurieva, {Rosa I.} and Lipkin, {Valery M.} and Zav'yalov, {Vladimir P.}",

note = "Funding Information: ☆ Supported by grants from the Russian Fundamental Science Foundation (97-04-49227) and International Science and Technology Center (Project 1462). ",

year = "2000",

month = mar,

doi = "10.1016/S0196-9781(00)00152-2",

language = "English (US)",

volume = "21",

pages = "353--357",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

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T1 - Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells

AU - Lepikhova, Tatyana N.

AU - Navolotskaya, Elena V.

AU - Zargarova, Tatyana A.

AU - Nurieva, Rosa I.

AU - Lipkin, Valery M.

AU - Zav'yalov, Vladimir P.

N1 - Funding Information: ☆ Supported by grants from the Russian Fundamental Science Foundation (97-04-49227) and International Science and Technology Center (Project 1462).

PY - 2000/3

Y1 - 2000/3

N2 - Influence of the ACTH-like peptide H-Val-Lys-Lys-Pro-Gly-Ser-Ser-Val- Lys-Val-OH corresponding to the sequence 11-20 of the variable part of human immunoglobulin G1 (IgG1) heavy chain on growth of MT-4 human T-lymphoblastoid cell line was investigated. It was found that the ACTH-like peptide at concentration range 10-11 -10-7 M inhibits the proliferation of MT-4 cells. Labeled ACTH 'address segment' [125I]ACTH (13-24) was used to establish that MT-4 cells express specific receptors for ACTH (K(d) = 97 pM). The ACTH-like peptide and human ACTH (but not IgG1 heavy chain) were shown to compete with [125I]ACTH (13-24) for binding to these receptors (K(i1) = 0.38 nM and K(i2) = 0.34 nM). (C) 2000 Elsevier Science Inc.

AB - Influence of the ACTH-like peptide H-Val-Lys-Lys-Pro-Gly-Ser-Ser-Val- Lys-Val-OH corresponding to the sequence 11-20 of the variable part of human immunoglobulin G1 (IgG1) heavy chain on growth of MT-4 human T-lymphoblastoid cell line was investigated. It was found that the ACTH-like peptide at concentration range 10-11 -10-7 M inhibits the proliferation of MT-4 cells. Labeled ACTH 'address segment' [125I]ACTH (13-24) was used to establish that MT-4 cells express specific receptors for ACTH (K(d) = 97 pM). The ACTH-like peptide and human ACTH (but not IgG1 heavy chain) were shown to compete with [125I]ACTH (13-24) for binding to these receptors (K(i1) = 0.38 nM and K(i2) = 0.34 nM). (C) 2000 Elsevier Science Inc.

KW - ACTH (Adrenocorticotropin)

KW - Growth inhibition

KW - MT-4 cells

KW - Peptide

KW - Receptor

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AN - SCOPUS:0033996947

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ER -

Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells

Abstract

Keywords

ASJC Scopus subject areas

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