TY - JOUR
T1 - Inhibition of early DNA-damage and chromosomal aberrations by Trianthema portulacastrum L. In carbon tetrachloride-induced mouse liver damage
AU - Sarkar, Alok
AU - Pradhan, Soumen
AU - Mukhopadhyay, Indranil
AU - Bose, Subrata K.
AU - Roy, Shyamal
AU - Chatterjee, Malay
N1 - Funding Information:
Dr Alok Sarkar is grateful to the Indian Council of Medical Research (ICMR, Govt. of India) for Research Associateship (Grant No. 3/2/3/75/97-NCD-III, Dated 8 June 1998). The cost of this work was partly defrayed from the Department of Science & Technology project, Govt of West Bengal, and is gratefully acknowledged.
PY - 1999/10
Y1 - 1999/10
N2 - The underlying molecular mechanisms of the antihepatotoxic activity of Trianthema portulacastrum by monitoring its effect on mouse liver DNA-chain break, sugar-base damage and chromosomal aberrations, during chronic or acute treatment with carbon tetrachloride (CCl4) have been studied. Daily oral feeding with the ethanolic extract (150 mg/kg basal diet, per os) was given 2 weeks before CCl4 treatment and continued until the end of the experiment (13 weeks). T. portulacastrum extract offer unique protection (P<0.05-0.001) against the induction of liver-specific structural-type chromosomal anomalies 15, 30 or 45 days after the last CCl4 insult, compared to control mice. This was further evidenced by extract-mediated protection (15 days prior feeding following a single necrogenic dose of CCl4) of the generation of DNA chain-break and Fe-sugar-base damage assays. The observed hepatoprotective mechanism could be due to its ability to counteract oxidative injury to DNA in the liver of mouse. (C) 1999 Academic Press.
AB - The underlying molecular mechanisms of the antihepatotoxic activity of Trianthema portulacastrum by monitoring its effect on mouse liver DNA-chain break, sugar-base damage and chromosomal aberrations, during chronic or acute treatment with carbon tetrachloride (CCl4) have been studied. Daily oral feeding with the ethanolic extract (150 mg/kg basal diet, per os) was given 2 weeks before CCl4 treatment and continued until the end of the experiment (13 weeks). T. portulacastrum extract offer unique protection (P<0.05-0.001) against the induction of liver-specific structural-type chromosomal anomalies 15, 30 or 45 days after the last CCl4 insult, compared to control mice. This was further evidenced by extract-mediated protection (15 days prior feeding following a single necrogenic dose of CCl4) of the generation of DNA chain-break and Fe-sugar-base damage assays. The observed hepatoprotective mechanism could be due to its ability to counteract oxidative injury to DNA in the liver of mouse. (C) 1999 Academic Press.
KW - Carbon tetrachloride
KW - Chromosomal aberrations
KW - DNA-chain break
KW - DNA-sugar-base damage
KW - Mouse liver
KW - Trianthema portulacastrum extract
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U2 - 10.1006/cbir.1999.0439
DO - 10.1006/cbir.1999.0439
M3 - Article
C2 - 10736194
AN - SCOPUS:0033510016
SN - 1065-6995
VL - 23
SP - 703
EP - 708
JO - Cell Biology International
JF - Cell Biology International
IS - 10
ER -