TY - JOUR
T1 - Inhibition of ras-induced proliferation and cellular transformation by p16INK4
AU - Serrano, Manuel
AU - Gómez-Lahoz, Enrique
AU - DePinho, Ronald A.
AU - Beach, David
AU - Bar-Sagi, Dafna
PY - 1995
Y1 - 1995
N2 - The cyclin-dependent kinase 4 (CDK4) regulates progression through the G1 phase of the cell cycle. The activity of CDK4 is controlled by the opposing effects of the D-type cyclin, an activating subunit, and p16 INK4, an inhibitory subunit. Ectopic expression of p16INK4 blocked entry into S phase of the cell cycle induced by oncogenic Ha-Ras, and this block was relieved by coexpression of a catalytically inactive CDK4 mutant. Expression of p16INK4 suppressed cellular transformation of primary rat embryo fibroblasts by oncogenic Ha-Ras and Myc, but not by Ha-Ras and E1a. Together, these observations provide direct evidence that p16INK4 can inhibit cell growth.
AB - The cyclin-dependent kinase 4 (CDK4) regulates progression through the G1 phase of the cell cycle. The activity of CDK4 is controlled by the opposing effects of the D-type cyclin, an activating subunit, and p16 INK4, an inhibitory subunit. Ectopic expression of p16INK4 blocked entry into S phase of the cell cycle induced by oncogenic Ha-Ras, and this block was relieved by coexpression of a catalytically inactive CDK4 mutant. Expression of p16INK4 suppressed cellular transformation of primary rat embryo fibroblasts by oncogenic Ha-Ras and Myc, but not by Ha-Ras and E1a. Together, these observations provide direct evidence that p16INK4 can inhibit cell growth.
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U2 - 10.1126/science.7809631
DO - 10.1126/science.7809631
M3 - Article
C2 - 7809631
AN - SCOPUS:0028931152
SN - 0036-8075
VL - 267
SP - 249
EP - 252
JO - Science
JF - Science
IS - 5195
ER -