Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors

Dong Chan Kim; So Young Lee; Dong Jae Jun; Sun Hee Kim; Jong Hee Lee; Eun Mi Hur; Nam In Baek; Kyong Tai Kim

doi:10.1016/j.bcp.2005.09.001

Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors

Dong Chan Kim, So Young Lee, Dong Jae Jun, Sun Hee Kim, Jong Hee Lee, Eun Mi Hur, Nam In Baek, Kyong Tai Kim

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Store-operated calcium entry (SOCE) plays an important role in shaping the Ca²⁺ response of various tissues and cell types. In this report, we show that thapsigargin (TG)-induced SOCE was inhibited by the histamine receptor agonist, histamine-trifluoromethyltoluide (HTMT), in U937 and HL-60 human promyelocytes. Preincubation of HTMT resulted in a significant inhibition of subsequent TG-induced Ca²⁺ elevation without affecting Ca ²⁺ release from intracellular stores. HTMT also inhibited TG-induced Ca²⁺ current and Ba²⁺/Mn²⁺ influx in a concentration-dependent manner. In contrast with HTMT, other H1 histamine receptor agonists, histamine, 2-methylhistamine and 2-thiazolylethylamine, did not affect TG-induced SOCE. In addition, HTMT also attenuated TG-induced cytosolic superoxide generation. Taken together, our data clearly suggest that the anti-inflammatory effect of HTMT may occur through direct inhibition of SOCE.

Original language	English (US)
Pages (from-to)	1613-1622
Number of pages	10
Journal	Biochemical Pharmacology
Volume	70
Issue number	11
DOIs	https://doi.org/10.1016/j.bcp.2005.09.001
State	Published - Nov 25 2005
Externally published	Yes

Keywords

Calcium
HTMT
Inflammation
SOCE
Superoxide

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/j.bcp.2005.09.001

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@article{37d1b952d1b0423ea1134adf65a5153b,

title = "Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors",

abstract = "Store-operated calcium entry (SOCE) plays an important role in shaping the Ca2+ response of various tissues and cell types. In this report, we show that thapsigargin (TG)-induced SOCE was inhibited by the histamine receptor agonist, histamine-trifluoromethyltoluide (HTMT), in U937 and HL-60 human promyelocytes. Preincubation of HTMT resulted in a significant inhibition of subsequent TG-induced Ca2+ elevation without affecting Ca 2+ release from intracellular stores. HTMT also inhibited TG-induced Ca2+ current and Ba2+/Mn2+ influx in a concentration-dependent manner. In contrast with HTMT, other H1 histamine receptor agonists, histamine, 2-methylhistamine and 2-thiazolylethylamine, did not affect TG-induced SOCE. In addition, HTMT also attenuated TG-induced cytosolic superoxide generation. Taken together, our data clearly suggest that the anti-inflammatory effect of HTMT may occur through direct inhibition of SOCE.",

keywords = "Calcium, HTMT, Inflammation, SOCE, Superoxide",

author = "Kim, {Dong Chan} and Lee, {So Young} and Jun, {Dong Jae} and Kim, {Sun Hee} and Lee, {Jong Hee} and Hur, {Eun Mi} and Baek, {Nam In} and Kim, {Kyong Tai}",

note = "Funding Information: We thanks to GlaxoSmithKline (Miss Lorraine Bray) Inc. for providing 2-methylhistamine and 2-thiazolylethylamine. This work was supported by the Frontier Research Project of the POSCO, Brain Korea 21 Program of the Korean Ministry of Education and BioGreen 21 program (Code# 20050401034641) of the Korean Rural Development Administration and the Brain Neurobiology Research Program (M10412000088-04N1200-08810).",

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doi = "10.1016/j.bcp.2005.09.001",

language = "English (US)",

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T1 - Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors

AU - Kim, Dong Chan

AU - Lee, So Young

AU - Jun, Dong Jae

AU - Kim, Sun Hee

AU - Lee, Jong Hee

AU - Hur, Eun Mi

AU - Baek, Nam In

AU - Kim, Kyong Tai

N1 - Funding Information: We thanks to GlaxoSmithKline (Miss Lorraine Bray) Inc. for providing 2-methylhistamine and 2-thiazolylethylamine. This work was supported by the Frontier Research Project of the POSCO, Brain Korea 21 Program of the Korean Ministry of Education and BioGreen 21 program (Code# 20050401034641) of the Korean Rural Development Administration and the Brain Neurobiology Research Program (M10412000088-04N1200-08810).

PY - 2005/11/25

Y1 - 2005/11/25

N2 - Store-operated calcium entry (SOCE) plays an important role in shaping the Ca2+ response of various tissues and cell types. In this report, we show that thapsigargin (TG)-induced SOCE was inhibited by the histamine receptor agonist, histamine-trifluoromethyltoluide (HTMT), in U937 and HL-60 human promyelocytes. Preincubation of HTMT resulted in a significant inhibition of subsequent TG-induced Ca2+ elevation without affecting Ca 2+ release from intracellular stores. HTMT also inhibited TG-induced Ca2+ current and Ba2+/Mn2+ influx in a concentration-dependent manner. In contrast with HTMT, other H1 histamine receptor agonists, histamine, 2-methylhistamine and 2-thiazolylethylamine, did not affect TG-induced SOCE. In addition, HTMT also attenuated TG-induced cytosolic superoxide generation. Taken together, our data clearly suggest that the anti-inflammatory effect of HTMT may occur through direct inhibition of SOCE.

AB - Store-operated calcium entry (SOCE) plays an important role in shaping the Ca2+ response of various tissues and cell types. In this report, we show that thapsigargin (TG)-induced SOCE was inhibited by the histamine receptor agonist, histamine-trifluoromethyltoluide (HTMT), in U937 and HL-60 human promyelocytes. Preincubation of HTMT resulted in a significant inhibition of subsequent TG-induced Ca2+ elevation without affecting Ca 2+ release from intracellular stores. HTMT also inhibited TG-induced Ca2+ current and Ba2+/Mn2+ influx in a concentration-dependent manner. In contrast with HTMT, other H1 histamine receptor agonists, histamine, 2-methylhistamine and 2-thiazolylethylamine, did not affect TG-induced SOCE. In addition, HTMT also attenuated TG-induced cytosolic superoxide generation. Taken together, our data clearly suggest that the anti-inflammatory effect of HTMT may occur through direct inhibition of SOCE.

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KW - HTMT

KW - Inflammation

KW - SOCE

KW - Superoxide

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Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors

Abstract

Keywords

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