Inhibition of the Mixed Lymphocyte Reaction with Fab Fragments from Anti-Human ALG

Ellen R. Richie; Michael T. Gallagher; Lyle R. Heim; Mary Ann South; John J. Trentin

doi:10.3181/00379727-140-36579

Inhibition of the Mixed Lymphocyte Reaction with Fab Fragments from Anti-Human ALG

Ellen R. Richie, Michael T. Gallagher, Lyle R. Heim, Mary Ann South, John J. Trentin

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The human mixed lymphocyte culture reaction (MLCR) as measured by incorporation of ¹⁴C-thymidine is inhibited in the presence of an appropriate concentration of equine anti-human lymphocyte globulin (ALG). Fab fragments prepared from ALG also inhibit the MLC reaction. However, unlike the intact ALG molecules, the Fab fragments are noncytotoxic for human lymphocytes. ALG derived Fab fragments could be potentially useful in suppressing allograft reactivity in vivo. We thank Dr. Gordon R. Dreesman for performing the analytical ultracentrifuge analyses and Mrs. Jane Ann Martin for her technical assistance.

Original language	English (US)
Pages (from-to)	916-919
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	140
Issue number	3
DOIs	https://doi.org/10.3181/00379727-140-36579
State	Published - Jul 1972
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Inhibition of the Mixed Lymphocyte Reaction with Fab Fragments from Anti-Human ALG",

abstract = "The human mixed lymphocyte culture reaction (MLCR) as measured by incorporation of 14C-thymidine is inhibited in the presence of an appropriate concentration of equine anti-human lymphocyte globulin (ALG). Fab fragments prepared from ALG also inhibit the MLC reaction. However, unlike the intact ALG molecules, the Fab fragments are noncytotoxic for human lymphocytes. ALG derived Fab fragments could be potentially useful in suppressing allograft reactivity in vivo. We thank Dr. Gordon R. Dreesman for performing the analytical ultracentrifuge analyses and Mrs. Jane Ann Martin for her technical assistance.",

author = "Richie, {Ellen R.} and Gallagher, {Michael T.} and Heim, {Lyle R.} and South, {Mary Ann} and Trentin, {John J.}",

note = "Funding Information: Supported by U.S. Public Health Service Grants T01-CA 05021, CA 03367, CA 12093, K6 CA 14219 A1 08227, K4-A1-23820.",

year = "1972",

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T1 - Inhibition of the Mixed Lymphocyte Reaction with Fab Fragments from Anti-Human ALG

AU - Richie, Ellen R.

AU - Gallagher, Michael T.

AU - Heim, Lyle R.

AU - South, Mary Ann

AU - Trentin, John J.

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PY - 1972/7

Y1 - 1972/7

N2 - The human mixed lymphocyte culture reaction (MLCR) as measured by incorporation of 14C-thymidine is inhibited in the presence of an appropriate concentration of equine anti-human lymphocyte globulin (ALG). Fab fragments prepared from ALG also inhibit the MLC reaction. However, unlike the intact ALG molecules, the Fab fragments are noncytotoxic for human lymphocytes. ALG derived Fab fragments could be potentially useful in suppressing allograft reactivity in vivo. We thank Dr. Gordon R. Dreesman for performing the analytical ultracentrifuge analyses and Mrs. Jane Ann Martin for her technical assistance.

AB - The human mixed lymphocyte culture reaction (MLCR) as measured by incorporation of 14C-thymidine is inhibited in the presence of an appropriate concentration of equine anti-human lymphocyte globulin (ALG). Fab fragments prepared from ALG also inhibit the MLC reaction. However, unlike the intact ALG molecules, the Fab fragments are noncytotoxic for human lymphocytes. ALG derived Fab fragments could be potentially useful in suppressing allograft reactivity in vivo. We thank Dr. Gordon R. Dreesman for performing the analytical ultracentrifuge analyses and Mrs. Jane Ann Martin for her technical assistance.

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Inhibition of the Mixed Lymphocyte Reaction with Fab Fragments from Anti-Human ALG

Abstract

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