Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein

Ratna B. Ray, Keith Meyer, Robert Steele, Anju Shrivastava, Bharat B. Aggarwal, Ranjit Ray

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) putative core protein has displayed many intriguing biological properties. Since tumor necrosis factor (TNF) plays an important role in controlling vital infection, in this study the effect of the core protein was investigated on the TNF-α induced apoptosis of human breast carcinoma cells (MCF7). HCV core protein when expressed inhibited TNF- α-induced apoptotic cell death unlike the control MCF7 cells, as determined by cell viability and DNA fragmentation analysis. Additionally, HCV core protein blocked the TNF-induced proteolytic cleavage of the death substrate poly(ADP-ribose) polymerase from its native 116-kDa protein to the characteristic 85-kDa polypeptide. Results from this study suggest that the HCV core protein plays a role in the inhibition of TNF-α-mediated cell death. Thus, the ability of core protein to inhibit the TNF-mediated apoptotic signaling pathway may provide a selective advantage for HCV replication, allowing for evasion of host antiviral defense mechanisms.

Original languageEnglish (US)
Pages (from-to)2256-2259
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number4
DOIs
StatePublished - Jan 23 1998

Fingerprint

Tumor Necrosis Factor-alpha
Apoptosis
MCF-7 Cells
Cell death
Viruses
Proteins
Cell Death
Cells
Poly(ADP-ribose) Polymerases
DNA Fragmentation
Virus Replication
Hepacivirus
Antiviral Agents
Hepatitis C virus nucleocapsid protein
Cell Survival
Breast Neoplasms
Peptides
DNA
Substrates
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein. / Ray, Ratna B.; Meyer, Keith; Steele, Robert; Shrivastava, Anju; Aggarwal, Bharat B.; Ray, Ranjit.

In: Journal of Biological Chemistry, Vol. 273, No. 4, 23.01.1998, p. 2256-2259.

Research output: Contribution to journalArticle

Ray, RB, Meyer, K, Steele, R, Shrivastava, A, Aggarwal, BB & Ray, R 1998, 'Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein', Journal of Biological Chemistry, vol. 273, no. 4, pp. 2256-2259. https://doi.org/10.1074/jbc.273.4.2256
Ray, Ratna B. ; Meyer, Keith ; Steele, Robert ; Shrivastava, Anju ; Aggarwal, Bharat B. ; Ray, Ranjit. / Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 4. pp. 2256-2259.
@article{1782499945374444b72475c0faeeff78,
title = "Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein",
abstract = "Hepatitis C virus (HCV) putative core protein has displayed many intriguing biological properties. Since tumor necrosis factor (TNF) plays an important role in controlling vital infection, in this study the effect of the core protein was investigated on the TNF-α induced apoptosis of human breast carcinoma cells (MCF7). HCV core protein when expressed inhibited TNF- α-induced apoptotic cell death unlike the control MCF7 cells, as determined by cell viability and DNA fragmentation analysis. Additionally, HCV core protein blocked the TNF-induced proteolytic cleavage of the death substrate poly(ADP-ribose) polymerase from its native 116-kDa protein to the characteristic 85-kDa polypeptide. Results from this study suggest that the HCV core protein plays a role in the inhibition of TNF-α-mediated cell death. Thus, the ability of core protein to inhibit the TNF-mediated apoptotic signaling pathway may provide a selective advantage for HCV replication, allowing for evasion of host antiviral defense mechanisms.",
author = "Ray, {Ratna B.} and Keith Meyer and Robert Steele and Anju Shrivastava and Aggarwal, {Bharat B.} and Ranjit Ray",
year = "1998",
month = "1",
day = "23",
doi = "10.1074/jbc.273.4.2256",
language = "English (US)",
volume = "273",
pages = "2256--2259",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "4",

}

TY - JOUR

T1 - Inhibition of tumor necrosis factor (TNF-α)-mediated apoptosis by hepatitis C virus core protein

AU - Ray, Ratna B.

AU - Meyer, Keith

AU - Steele, Robert

AU - Shrivastava, Anju

AU - Aggarwal, Bharat B.

AU - Ray, Ranjit

PY - 1998/1/23

Y1 - 1998/1/23

N2 - Hepatitis C virus (HCV) putative core protein has displayed many intriguing biological properties. Since tumor necrosis factor (TNF) plays an important role in controlling vital infection, in this study the effect of the core protein was investigated on the TNF-α induced apoptosis of human breast carcinoma cells (MCF7). HCV core protein when expressed inhibited TNF- α-induced apoptotic cell death unlike the control MCF7 cells, as determined by cell viability and DNA fragmentation analysis. Additionally, HCV core protein blocked the TNF-induced proteolytic cleavage of the death substrate poly(ADP-ribose) polymerase from its native 116-kDa protein to the characteristic 85-kDa polypeptide. Results from this study suggest that the HCV core protein plays a role in the inhibition of TNF-α-mediated cell death. Thus, the ability of core protein to inhibit the TNF-mediated apoptotic signaling pathway may provide a selective advantage for HCV replication, allowing for evasion of host antiviral defense mechanisms.

AB - Hepatitis C virus (HCV) putative core protein has displayed many intriguing biological properties. Since tumor necrosis factor (TNF) plays an important role in controlling vital infection, in this study the effect of the core protein was investigated on the TNF-α induced apoptosis of human breast carcinoma cells (MCF7). HCV core protein when expressed inhibited TNF- α-induced apoptotic cell death unlike the control MCF7 cells, as determined by cell viability and DNA fragmentation analysis. Additionally, HCV core protein blocked the TNF-induced proteolytic cleavage of the death substrate poly(ADP-ribose) polymerase from its native 116-kDa protein to the characteristic 85-kDa polypeptide. Results from this study suggest that the HCV core protein plays a role in the inhibition of TNF-α-mediated cell death. Thus, the ability of core protein to inhibit the TNF-mediated apoptotic signaling pathway may provide a selective advantage for HCV replication, allowing for evasion of host antiviral defense mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=0031892177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031892177&partnerID=8YFLogxK

U2 - 10.1074/jbc.273.4.2256

DO - 10.1074/jbc.273.4.2256

M3 - Article

C2 - 9442069

AN - SCOPUS:0031892177

VL - 273

SP - 2256

EP - 2259

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 4

ER -