Integration of optical devices and nanotechnology for conducting genome research

SPR based sensing techniques utilize a spectroscopy for transducing biomolecular binding events to variations in spectra. This label-free and real-time technique has widely applied for conducting biomedical research. In this study, we present a spectroscopy-based SPR system for monitoring binding between human serum albumin and nucleic acid library. Compared with conventional SPR technique, this novel system utilizes cost-effective nanostructured arrays and a portable UV-Vis spectrometer. These advantages enable a promising development of a portable analytical device for widespread applications. Meanwhile, multispectral analysis used here also helps increase the sensitivity, and thus transducing the binding event to optical signal efficiently. The result demonstrates that this cost-effective and portable system could be applied for a future application of selecting target aptamer. Moreover, we also present surface enhanced Raman spectroscopy (SERS) on the nanostructured arrays in a label-free approach. This integration of multiple spectroscopy technologies is utilized for conducting genome research efficiently.