Interaction between Cdc37 and Cdk4 in human cells

Lou Lamphere; Francesca Fiore; Xu Xu; Leonardo Brizuela; Susan Keezer; Claude Sardet; Giulio F. Draetta; Jeno Gyuris

doi:10.1038/sj.onc.1201036

Interaction between Cdc37 and Cdk4 in human cells

Lou Lamphere, Francesca Fiore, Xu Xu, Leonardo Brizuela, Susan Keezer, Claude Sardet, Giulio F. Draetta, Jeno Gyuris

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Using the yeast two-hybrid system we have identified novel potential Cdk4 interacting proteins. Here we described the interaction Cdk4 with a human homologue of the yeast Drosophila CDC37 gene products. Cdc37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. Cdc37 is not an inhibitor nor an activator of the Cdk4/cyclin D1 kinase, while it appears to facilitate complex assembly between Cdk4 and cyclin D1 in vitro. Cdc37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via Cdc37.

Original language	English (US)
Pages (from-to)	1999-2004
Number of pages	6
Journal	Oncogene
Volume	14
Issue number	16
DOIs	https://doi.org/10.1038/sj.onc.1201036
State	Published - 1997
Externally published	Yes

Keywords

Cdc37
Cdk
Cyclin
Hsp90

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1201036

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title = "Interaction between Cdc37 and Cdk4 in human cells",

abstract = "Using the yeast two-hybrid system we have identified novel potential Cdk4 interacting proteins. Here we described the interaction Cdk4 with a human homologue of the yeast Drosophila CDC37 gene products. Cdc37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. Cdc37 is not an inhibitor nor an activator of the Cdk4/cyclin D1 kinase, while it appears to facilitate complex assembly between Cdk4 and cyclin D1 in vitro. Cdc37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via Cdc37.",

keywords = "Cdc37, Cdk, Cyclin, Hsp90",

author = "Lou Lamphere and Francesca Fiore and Xu Xu and Leonardo Brizuela and Susan Keezer and Claude Sardet and Draetta, {Giulio F.} and Jeno Gyuris",

year = "1997",

doi = "10.1038/sj.onc.1201036",

language = "English (US)",

volume = "14",

pages = "1999--2004",

journal = "Oncogene",

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T1 - Interaction between Cdc37 and Cdk4 in human cells

AU - Lamphere, Lou

AU - Fiore, Francesca

AU - Xu, Xu

AU - Brizuela, Leonardo

AU - Keezer, Susan

AU - Sardet, Claude

AU - Draetta, Giulio F.

AU - Gyuris, Jeno

PY - 1997

Y1 - 1997

N2 - Using the yeast two-hybrid system we have identified novel potential Cdk4 interacting proteins. Here we described the interaction Cdk4 with a human homologue of the yeast Drosophila CDC37 gene products. Cdc37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. Cdc37 is not an inhibitor nor an activator of the Cdk4/cyclin D1 kinase, while it appears to facilitate complex assembly between Cdk4 and cyclin D1 in vitro. Cdc37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via Cdc37.

AB - Using the yeast two-hybrid system we have identified novel potential Cdk4 interacting proteins. Here we described the interaction Cdk4 with a human homologue of the yeast Drosophila CDC37 gene products. Cdc37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. Cdc37 is not an inhibitor nor an activator of the Cdk4/cyclin D1 kinase, while it appears to facilitate complex assembly between Cdk4 and cyclin D1 in vitro. Cdc37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via Cdc37.

KW - Cdc37

KW - Cdk

KW - Cyclin

KW - Hsp90

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DO - 10.1038/sj.onc.1201036

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AN - SCOPUS:0030998294

SN - 0950-9232

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JO - Oncogene

JF - Oncogene

IS - 16

ER -

Interaction between Cdc37 and Cdk4 in human cells

Abstract

Keywords

ASJC Scopus subject areas

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