Interaction between GNB3 C825T and ACE I/D polymorphisms in pre-eclampsia

Objective: To verify the hypothesis if interaction between the G protein β3 subunit (GNB3) C825T polymorphism and angiotensin-I converting enzyme (ACE) insertion/deletion (I/D) could lead to the increased risk of pre-eclampsia. Methods: Analyses of ACE and GNB3 genotypes were performed in 188 preeclamptic patients and 273 normal pregnant controls by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism in Chinese population, respectively. Results: The distributions of alleles and genotypes for the GNB3 C825T and ACE I/D polymorphisms were not found to be significantly associathed with pre-eclamptic status. No significant interaction of the influence of GNB3 T allele and ACE genotypes on the risk of pre-eclampsia was observed (OR 0. 439-1. 203, all P>0. 05). However, we found that in homozygous 825T genotype carriers with the ACE II genotype in controls diastolic blood pressure (DBP) levels showed highest [(77. 61±1. 26) mmHg (1 mmHg=0. 133 kPa)] among other three genotype combinations [TT/ID, (70.94± 1. 64) mmHg; CT/ID, (73. 15±0. 89) mmHg; CT/DD, (72. 57±2. 14) mmHg] (all P<0. 05). No significant effect on systolic blood pressure (SBP) or DBP levels in the patients were observed. Conclusion: Our data suggest no significant interaction of the GNB3 825T allele carriers with the ACE I/D polymorphism in pre-eclampsia in Chinese population in Chengdu area. However there is the interaction of the two genes on DBP levels in pregnancy women without pre-eclampsia in the population.