TY - JOUR
T1 - Interactions of a Rel protein with its inhibitor
AU - Lehming, Norbert
AU - Mcguire, Sean
AU - Brickman, Joshua M.
AU - Ptashne, Mark
PY - 1995/10/24
Y1 - 1995/10/24
N2 - Cactus, a Drosophila homologue of IκB, binds to and inhibits Dorsal, a homologue of the p50 and p65 components of NF-κB. We describe experiments in yeast with various Dorsal and Cactus derivatives showing that Cactus blocks the DNA binding and nuclear localization functions of Dorsal. In contrast, Dorsal's transcriptional activating region is functional in the Dorsal- Cactus complex. We identify two Dorsal mutants, Dorsal C233R and Dorsal S234P, that escape Cactus inhibition in vivo, and we show that these mutants fail to interact with Cactus in vitro. From this and data of others, we identify the likely surface of Dorsal that binds Cactus. We also describe a modified PCR mutagenesis procedure, easier to use than conventional methods, that produces a library of high complexity.
AB - Cactus, a Drosophila homologue of IκB, binds to and inhibits Dorsal, a homologue of the p50 and p65 components of NF-κB. We describe experiments in yeast with various Dorsal and Cactus derivatives showing that Cactus blocks the DNA binding and nuclear localization functions of Dorsal. In contrast, Dorsal's transcriptional activating region is functional in the Dorsal- Cactus complex. We identify two Dorsal mutants, Dorsal C233R and Dorsal S234P, that escape Cactus inhibition in vivo, and we show that these mutants fail to interact with Cactus in vitro. From this and data of others, we identify the likely surface of Dorsal that binds Cactus. We also describe a modified PCR mutagenesis procedure, easier to use than conventional methods, that produces a library of high complexity.
KW - Rel domain
KW - ankyrin repeats
KW - protein-protein interaction
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U2 - 10.1073/pnas.92.22.10242
DO - 10.1073/pnas.92.22.10242
M3 - Article
C2 - 7479760
AN - SCOPUS:0028826019
SN - 0027-8424
VL - 92
SP - 10242
EP - 10246
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -