Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients

O. M.Zack Howard; Moshe Talpaz; Hagop Kantarjian; David Seong; Andrzej Wedrychowski; Nikolaos Paslidis; Jeane Hester; Anne Cork; James Turpin; Gabriel Lopez-Berestein; Jose Trujillo; Jordan Gutterman; Emil Freireich; Albert Deisseroth

Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients

O. M.Zack Howard, Moshe Talpaz, Hagop Kantarjian, David Seong, Andrzej Wedrychowski, Nikolaos Paslidis, Jeane Hester, Anne Cork, James Turpin, Gabriel Lopez-Berestein, Jose Trujillo, Jordan Gutterman, Emil Freireich, Albert Deisseroth

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells contained an activity that altered the electrophoretic mobility of complexes formed between nuclear proteins and the transcriptional enhancers of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-α diminished the effect of the CML cytoplasmic proteins on these nuclear protein-DNA complexes. The presence of clinical responsiveness to IFN-α correlated with the sensitivity to the IFN-induced change in the electrophoretic mobility of nuclear protein-DNA complexes. These data suggest that the action of IFN-α in CML may be linked to a pathway that can result in posttranslational modification of nuclear proteins.

Original language	English (US)
Pages (from-to)	1117-1130
Number of pages	14
Journal	Blood
Volume	76
Issue number	6
State	Published - Sep 15 1990

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

Cite this

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title = "Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients",

abstract = "Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells contained an activity that altered the electrophoretic mobility of complexes formed between nuclear proteins and the transcriptional enhancers of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-α diminished the effect of the CML cytoplasmic proteins on these nuclear protein-DNA complexes. The presence of clinical responsiveness to IFN-α correlated with the sensitivity to the IFN-induced change in the electrophoretic mobility of nuclear protein-DNA complexes. These data suggest that the action of IFN-α in CML may be linked to a pathway that can result in posttranslational modification of nuclear proteins.",

author = "Howard, {O. M.Zack} and Moshe Talpaz and Hagop Kantarjian and David Seong and Andrzej Wedrychowski and Nikolaos Paslidis and Jeane Hester and Anne Cork and James Turpin and Gabriel Lopez-Berestein and Jose Trujillo and Jordan Gutterman and Emil Freireich and Albert Deisseroth",

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language = "English (US)",

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pages = "1117--1130",

journal = "Blood",

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T1 - Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients

AU - Howard, O. M.Zack

AU - Talpaz, Moshe

AU - Kantarjian, Hagop

AU - Seong, David

AU - Wedrychowski, Andrzej

AU - Paslidis, Nikolaos

AU - Hester, Jeane

AU - Cork, Anne

AU - Turpin, James

AU - Lopez-Berestein, Gabriel

AU - Trujillo, Jose

AU - Gutterman, Jordan

AU - Freireich, Emil

AU - Deisseroth, Albert

PY - 1990/9/15

Y1 - 1990/9/15

N2 - Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells contained an activity that altered the electrophoretic mobility of complexes formed between nuclear proteins and the transcriptional enhancers of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-α diminished the effect of the CML cytoplasmic proteins on these nuclear protein-DNA complexes. The presence of clinical responsiveness to IFN-α correlated with the sensitivity to the IFN-induced change in the electrophoretic mobility of nuclear protein-DNA complexes. These data suggest that the action of IFN-α in CML may be linked to a pathway that can result in posttranslational modification of nuclear proteins.

AB - Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells contained an activity that altered the electrophoretic mobility of complexes formed between nuclear proteins and the transcriptional enhancers of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-α diminished the effect of the CML cytoplasmic proteins on these nuclear protein-DNA complexes. The presence of clinical responsiveness to IFN-α correlated with the sensitivity to the IFN-induced change in the electrophoretic mobility of nuclear protein-DNA complexes. These data suggest that the action of IFN-α in CML may be linked to a pathway that can result in posttranslational modification of nuclear proteins.

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Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients

Abstract

ASJC Scopus subject areas

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