Abstract
We report that recombinant human interleukin-4 (IL-4) downregulates interleukin-6 (IL-6) production by human peripheral blood mononuclear cells (PBMC). PBMC were preincubated for up to 24 hr in the presence of IL-4 (100 U/ml) and then activated with lipopolysaccharide B Escherichia coli 026:B6 (LPS, 10 μg/ml), recombinant human tumor necrosis factor-α (TNF-α, 200 U/ml), or Concanavalin A (Con A, 10 μg/ml). Although all these signals induced IL-6 production, IL-4-treated cells produced significantly reduced levels of IL-6 protein. This effect was dose and time dependent. We conclude that IL-4 is a potent downregulatory modulator of IL-6 expression in human PBMC.
Original language | English (US) |
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Pages (from-to) | 475-479 |
Number of pages | 5 |
Journal | Journal of Leukocyte Biology |
Volume | 47 |
Issue number | 5 |
DOIs | |
State | Published - 1990 |
Externally published | Yes |
Keywords
- B-cell stimulatory factor-1
- TNF-α
- regulation of IL-6
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology