Abstract
Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.
Original language | English (US) |
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Pages (from-to) | 2897-2912 |
Number of pages | 16 |
Journal | Nucleic acids research |
Volume | 16 |
Issue number | 7 |
DOIs | |
State | Published - Apr 11 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics