TY - JOUR
T1 - Interrogating cellular communication in cancer with genetically encoded imaging reporters
AU - Gammon, Seth T.
AU - Liu, Tracy W.
AU - Piwnica-Worms, David
N1 - Funding Information:
Work was funded by a grant to the Washington University-MD Anderson Cancer Center Inter-Institutional Molecular Imaging Center (National Cancer Institute P50 CA94056), an Odyssey Fellowship to T.W.L., and a Canadian Institute of Health Research Postdoctoral Fellowship to T.W.L.
Funding Information:
institution received grant from National Cancer Institute (Washington University-MD Anderson Cancer Center Inter-Institutional Molecular Imaging Center (NCI P50 CA94056). Activities not related to the present article: disclosed no relevant relationships. Other relationships: disclosed no relevant relationships. T.W.L. disclosed no relevant relationships. D.P.W. disclosed no relevant relationships.
Publisher Copyright:
© RSNA, 2020.
PY - 2020/7
Y1 - 2020/7
N2 - Cells continuously communicate changes in their microenvironment, both locally and globally, with other cells in the organism. Integration of information arising from signaling networks impart continuous, time-dependent changes of cell function and pheno-type. Use of genetically encoded reporters enable researchers to noninvasively monitor time-dependent changes in intercellular and intracellular signaling, which can be interrogated by macroscopic and microscopic optical imaging, nuclear medicine imaging, MRI, and even photoacoustic imaging techniques. Reporters enable noninvasive monitoring of changes in cell-to-cell proximity, transcription, translation, protein folding, protein association, protein degradation, drug action, and second messengers in real time. Because of their positive impact on preclinical research, attempts to improve the sensitivity and specificity of these reporters, and to develop new types and classes of reporters, remain an active area of investigation. A few reporters have migrated to proof-of-principle clinical demonstra-tions, and recent advances in genome editing technologies may enable the use of reporters in the context of genome-wide analysis and the imaging of complex genomic regulation in vivo that cannot be readily investigated through standard methodologies. The combination of genetically encoded imaging reporters with continuous improvements in other molecular biology techniques may enhance and expedite target discovery and drug development for cancer interventions and treatment.
AB - Cells continuously communicate changes in their microenvironment, both locally and globally, with other cells in the organism. Integration of information arising from signaling networks impart continuous, time-dependent changes of cell function and pheno-type. Use of genetically encoded reporters enable researchers to noninvasively monitor time-dependent changes in intercellular and intracellular signaling, which can be interrogated by macroscopic and microscopic optical imaging, nuclear medicine imaging, MRI, and even photoacoustic imaging techniques. Reporters enable noninvasive monitoring of changes in cell-to-cell proximity, transcription, translation, protein folding, protein association, protein degradation, drug action, and second messengers in real time. Because of their positive impact on preclinical research, attempts to improve the sensitivity and specificity of these reporters, and to develop new types and classes of reporters, remain an active area of investigation. A few reporters have migrated to proof-of-principle clinical demonstra-tions, and recent advances in genome editing technologies may enable the use of reporters in the context of genome-wide analysis and the imaging of complex genomic regulation in vivo that cannot be readily investigated through standard methodologies. The combination of genetically encoded imaging reporters with continuous improvements in other molecular biology techniques may enhance and expedite target discovery and drug development for cancer interventions and treatment.
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U2 - 10.1148/rycan.2020190053
DO - 10.1148/rycan.2020190053
M3 - Review article
C2 - 32803164
AN - SCOPUS:85115896869
SN - 2638-616X
VL - 2
JO - Radiology: Imaging Cancer
JF - Radiology: Imaging Cancer
IS - 4
M1 - e190053
ER -