Intravital imaging of mouse embryos

Qiang Huang; Malkiel A. Cohen; Fernando C. Alsina; Garth Devlin; Aliesha Garrett; Jennifer McKey; Patrick Havlik; Nikolai Rakhilin; Ergang Wang; Kun Xiang; Parker Mathews; Lihua Wang; Cheryl Bock; Victor Ruthig; Yi Wang; Marcos Negrete; Chi Wut Wong; Preetish K.L. Murthy; Shupei Zhang; Andrea R. Daniel; David G. Kirsch; Yubin Kang; Blanche Capel; Aravind Asokan; Debra L. Silver; Rudolf Jaenisch; Xiling Shen

doi:10.1126/science.aba0210

Intravital imaging of mouse embryos

Qiang Huang, Malkiel A. Cohen, Fernando C. Alsina, Garth Devlin, Aliesha Garrett, Jennifer McKey, Patrick Havlik, Nikolai Rakhilin, Ergang Wang, Kun Xiang, Parker Mathews, Lihua Wang, Cheryl Bock, Victor Ruthig, Yi Wang, Marcos Negrete, Chi Wut Wong, Preetish K.L. Murthy, Shupei Zhang, Andrea R. DanielDavid G. Kirsch, Yubin Kang, Blanche Capel, Aravind Asokan, Debra L. Silver, Rudolf Jaenisch, Xiling Shen

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.

Original language	English (US)
Pages (from-to)	181-186
Number of pages	6
Journal	Science
Volume	368
Issue number	6487
DOIs	https://doi.org/10.1126/science.aba0210
State	Published - Apr 10 2020
Externally published	Yes

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Huang, Q, Cohen, MA, Alsina, FC, Devlin, G, Garrett, A, McKey, J, Havlik, P, Rakhilin, N, Wang, E, Xiang, K, Mathews, P, Wang, L, Bock, C, Ruthig, V, Wang, Y, Negrete, M, Wong, CW, Murthy, PKL, Zhang, S, Daniel, AR, Kirsch, DG, Kang, Y, Capel, B, Asokan, A, Silver, DL, Jaenisch, R & Shen, X 2020, 'Intravital imaging of mouse embryos', Science, vol. 368, no. 6487, pp. 181-186. https://doi.org/10.1126/science.aba0210

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title = "Intravital imaging of mouse embryos",

abstract = "Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.",

author = "Qiang Huang and Cohen, {Malkiel A.} and Alsina, {Fernando C.} and Garth Devlin and Aliesha Garrett and Jennifer McKey and Patrick Havlik and Nikolai Rakhilin and Ergang Wang and Kun Xiang and Parker Mathews and Lihua Wang and Cheryl Bock and Victor Ruthig and Yi Wang and Marcos Negrete and Wong, {Chi Wut} and Murthy, {Preetish K.L.} and Shupei Zhang and Daniel, {Andrea R.} and Kirsch, {David G.} and Yubin Kang and Blanche Capel and Aravind Asokan and Silver, {Debra L.} and Rudolf Jaenisch and Xiling Shen",

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doi = "10.1126/science.aba0210",

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AU - Huang, Qiang

AU - Cohen, Malkiel A.

AU - Alsina, Fernando C.

AU - Devlin, Garth

AU - Garrett, Aliesha

AU - McKey, Jennifer

AU - Havlik, Patrick

AU - Rakhilin, Nikolai

AU - Wang, Ergang

AU - Xiang, Kun

AU - Mathews, Parker

AU - Wang, Lihua

AU - Bock, Cheryl

AU - Ruthig, Victor

AU - Wang, Yi

AU - Negrete, Marcos

AU - Wong, Chi Wut

AU - Murthy, Preetish K.L.

AU - Zhang, Shupei

AU - Daniel, Andrea R.

AU - Kirsch, David G.

AU - Kang, Yubin

AU - Capel, Blanche

AU - Asokan, Aravind

AU - Silver, Debra L.

AU - Jaenisch, Rudolf

AU - Shen, Xiling

PY - 2020/4/10

Y1 - 2020/4/10

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AB - Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.

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Intravital imaging of mouse embryos

Abstract

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