TY - JOUR
T1 - Inverse relationship between TCTP/RhoA and p53//cyclin A/actin expression in ovarian cancer cells
AU - Kloc, Malgorzata
AU - Tejpal, Neelam
AU - Sidhu, Jitinderpal
AU - Ganachari, Malathesha
AU - Flores-Villanueva, Pedro
AU - Jennings, Nicholas B.
AU - Sood, Anil K.
AU - Kubiak, Jacek Z.
AU - Ghobrial, Rafik M.
PY - 2012
Y1 - 2012
N2 - The translationally controlled tumor protein (TCTP) plays a role in cell growth, cell cycle and cancer progression. TCTP controls negatively the stability of the p53 tumor suppressor protein and interacts with the cellular cytoskeleton. The deregulation of the actin and cytokeratin cytoskeleton is responsible for the increased migratory activity of tumor cells and is linked with poor patient outcome. Recent studies indicate that cyclin A, a key regulator of cell cycle, controls actin organization and negatively regulates cell motility via regulation of RhoA expression. We studied the organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53, cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 (expressing low level of inducible p53) ovarian epithelial cancer cells with different metastatic potential. Immunostainingand ultrastructural analyses illustrated a dramatic difference in the organization of the cytokeratin and actin filaments in non-transformed versus cancer cell lines. We also determined that there is an inverse relationship between the level of TCTP/RhoA and actin/p53/cyclin A expression in ovarian cancer cell lines. This previously unidentified negative relationship between TCTP/RhoA and actin/p53/cyclin A may suggest that this inte raction is linked with the high aggressiveness of ovarian cancers.
AB - The translationally controlled tumor protein (TCTP) plays a role in cell growth, cell cycle and cancer progression. TCTP controls negatively the stability of the p53 tumor suppressor protein and interacts with the cellular cytoskeleton. The deregulation of the actin and cytokeratin cytoskeleton is responsible for the increased migratory activity of tumor cells and is linked with poor patient outcome. Recent studies indicate that cyclin A, a key regulator of cell cycle, controls actin organization and negatively regulates cell motility via regulation of RhoA expression. We studied the organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53, cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 (expressing low level of inducible p53) ovarian epithelial cancer cells with different metastatic potential. Immunostainingand ultrastructural analyses illustrated a dramatic difference in the organization of the cytokeratin and actin filaments in non-transformed versus cancer cell lines. We also determined that there is an inverse relationship between the level of TCTP/RhoA and actin/p53/cyclin A expression in ovarian cancer cell lines. This previously unidentified negative relationship between TCTP/RhoA and actin/p53/cyclin A may suggest that this inte raction is linked with the high aggressiveness of ovarian cancers.
KW - Actin
KW - Cyclin A
KW - Cytokeratin
KW - Ovarian cancer cell lines
KW - P53
KW - RhoA
KW - TCTP
UR - http://www.scopus.com/inward/record.url?scp=84867808831&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867808831&partnerID=8YFLogxK
U2 - 10.5603/FHC.2012.0049
DO - 10.5603/FHC.2012.0049
M3 - Article
C2 - 23042265
AN - SCOPUS:84867808831
SN - 0239-8508
VL - 50
SP - 358
EP - 367
JO - Folia Histochemica et Cytobiologica
JF - Folia Histochemica et Cytobiologica
IS - 3
ER -