TY - JOUR
T1 - Investigation on sodium valproate induced germ cell damage, oxidative stress and genotoxicity in male Swiss mice
AU - Khan, Sabbir
AU - Ahmad, Tauseef
AU - Parekh, Chintan Vishnubhai
AU - Trivedi, Priyanka Pushkarbhai
AU - Kushwaha, Sapana
AU - Jena, Gopabandhu
N1 - Funding Information:
We wish to acknowledge the financial assistance received from National Institute of Pharmaceutical Education and Research (NIPER), Mohali to undertake the present study.
PY - 2011/12
Y1 - 2011/12
N2 - Sodium valproate (VPA) is the most widely used antiepileptic drug for the treatment of epilepsy, bipolar psychiatric disorders and migraine. However, long-term VPA treatment has several adverse effects on the reproductive system. The present study was aimed to investigate the possible germ cell toxicity of VPA in mice. Animals were treated with VPA intraperitoneally for 10 and 28 days at the doses of 500. mg/kg-d and 100, 200 and 400. mg/kg-d, respectively, and were sacrificed 24. h after the last dose. The germ cell toxicity of VPA was assessed using oxidative stress parameters, sperm count, sperm head morphology, sperm comet assay, 8-oxo-dG expression and histology. VPA treatment significantly decreased the sperm count, testes and epididymis weight and significantly increased the sperm head abnormality, sperm DNA damage, oxidative stress and 8-oxo-dG expression in the testes of mice. The present study illustrates that VPA induced germ cell toxicity in mice.
AB - Sodium valproate (VPA) is the most widely used antiepileptic drug for the treatment of epilepsy, bipolar psychiatric disorders and migraine. However, long-term VPA treatment has several adverse effects on the reproductive system. The present study was aimed to investigate the possible germ cell toxicity of VPA in mice. Animals were treated with VPA intraperitoneally for 10 and 28 days at the doses of 500. mg/kg-d and 100, 200 and 400. mg/kg-d, respectively, and were sacrificed 24. h after the last dose. The germ cell toxicity of VPA was assessed using oxidative stress parameters, sperm count, sperm head morphology, sperm comet assay, 8-oxo-dG expression and histology. VPA treatment significantly decreased the sperm count, testes and epididymis weight and significantly increased the sperm head abnormality, sperm DNA damage, oxidative stress and 8-oxo-dG expression in the testes of mice. The present study illustrates that VPA induced germ cell toxicity in mice.
KW - Genotoxicity
KW - Germ cell
KW - Mice
KW - Oxidative stress
KW - Sodium valproate
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U2 - 10.1016/j.reprotox.2011.09.007
DO - 10.1016/j.reprotox.2011.09.007
M3 - Article
C2 - 22001255
AN - SCOPUS:80055014296
SN - 0890-6238
VL - 32
SP - 385
EP - 394
JO - Reproductive Toxicology
JF - Reproductive Toxicology
IS - 4
ER -