Isolation and Characterization of a Rat Luteal cDNA Encoding 20α-Hydroxysteroid Dehydrogenase

J. Mao; W. R. Duan; C. T. Albarracin; T. G. Parmer; G. Gibori

doi:10.1006/bbrc.1994.1844

Isolation and Characterization of a Rat Luteal cDNA Encoding 20α-Hydroxysteroid Dehydrogenase

J. Mao, W. R. Duan, C. T. Albarracin, T. G. Parmer, G. Gibori

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Abstract

We report the isolation and characterization of a full length cDNA encoding rat 20αhydroxysteroid dehydrogenase derived from rat corpus luteum RNA. The predicted amino acid sequence of the protein encoded by the 20αHSD clone is composed of 323 amino acids possessing an approximate molecular weight of 37 kDa. The sequence of peptides derived from the purified protein was found in the translated sequence of the open reading frame. cDNA and amino acid sequence indicate that the rat ovarian 20αHSD belongs to the aldo-keto reductase family of enzymes. Northern analysis revealed a 1.2 Kb 20αHSD mRNA in corpora lutea undergoing luteolysis. Prolactin reduced markedly 20αHSD mRNA expression. No signal was detected in other tissues examined, demonstrating the specific expression of this enzyme in the corpus luteum.

Original language	English (US)
Pages (from-to)	1289-1295
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	201
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1994.1844
State	Published - Jun 30 1994
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Isolation and Characterization of a Rat Luteal cDNA Encoding 20α-Hydroxysteroid Dehydrogenase",

abstract = "We report the isolation and characterization of a full length cDNA encoding rat 20αhydroxysteroid dehydrogenase derived from rat corpus luteum RNA. The predicted amino acid sequence of the protein encoded by the 20αHSD clone is composed of 323 amino acids possessing an approximate molecular weight of 37 kDa. The sequence of peptides derived from the purified protein was found in the translated sequence of the open reading frame. cDNA and amino acid sequence indicate that the rat ovarian 20αHSD belongs to the aldo-keto reductase family of enzymes. Northern analysis revealed a 1.2 Kb 20αHSD mRNA in corpora lutea undergoing luteolysis. Prolactin reduced markedly 20αHSD mRNA expression. No signal was detected in other tissues examined, demonstrating the specific expression of this enzyme in the corpus luteum.",

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T1 - Isolation and Characterization of a Rat Luteal cDNA Encoding 20α-Hydroxysteroid Dehydrogenase

AU - Mao, J.

AU - Duan, W. R.

AU - Albarracin, C. T.

AU - Parmer, T. G.

AU - Gibori, G.

PY - 1994/6/30

Y1 - 1994/6/30

N2 - We report the isolation and characterization of a full length cDNA encoding rat 20αhydroxysteroid dehydrogenase derived from rat corpus luteum RNA. The predicted amino acid sequence of the protein encoded by the 20αHSD clone is composed of 323 amino acids possessing an approximate molecular weight of 37 kDa. The sequence of peptides derived from the purified protein was found in the translated sequence of the open reading frame. cDNA and amino acid sequence indicate that the rat ovarian 20αHSD belongs to the aldo-keto reductase family of enzymes. Northern analysis revealed a 1.2 Kb 20αHSD mRNA in corpora lutea undergoing luteolysis. Prolactin reduced markedly 20αHSD mRNA expression. No signal was detected in other tissues examined, demonstrating the specific expression of this enzyme in the corpus luteum.

AB - We report the isolation and characterization of a full length cDNA encoding rat 20αhydroxysteroid dehydrogenase derived from rat corpus luteum RNA. The predicted amino acid sequence of the protein encoded by the 20αHSD clone is composed of 323 amino acids possessing an approximate molecular weight of 37 kDa. The sequence of peptides derived from the purified protein was found in the translated sequence of the open reading frame. cDNA and amino acid sequence indicate that the rat ovarian 20αHSD belongs to the aldo-keto reductase family of enzymes. Northern analysis revealed a 1.2 Kb 20αHSD mRNA in corpora lutea undergoing luteolysis. Prolactin reduced markedly 20αHSD mRNA expression. No signal was detected in other tissues examined, demonstrating the specific expression of this enzyme in the corpus luteum.

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Isolation and Characterization of a Rat Luteal cDNA Encoding 20α-Hydroxysteroid Dehydrogenase

Abstract

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