@article{36925e3e2e064f1293213390ddcdbb73,
title = "Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries",
abstract = "We have identified and mapped 61 novel and previously described chromosome 17 and X genes, using a human placental cDNA library. These genes were isolated using a gene identification and mapping strategy based on reciprocal probing of arrayed chromosome specific cosmid and cDNA libraries. This strategy scans gridded cosmids for nuclear genes and isolates the expressed sequence by a cosmid to cDNA filter hybridization. Inherent to this approach is the advantage of identifying the corresponding genomic cosmid clone of a particular cDNA. The genomic and cDNA reagents can be used for fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) based mapping to resolve map positions of cDNAs belonging to gene families and those associated with multiple chromosomes. The downstream utility of reagents generated by the reciprocal probing methods is demonstrated in our studies.",
author = "Lee, {Cheng Chi} and Ali Yazdani and Manfred Wehnert and Zhaoyang Zhao and Lindsay, {Elizabeth A.} and Jennifer Bailey and Coolbaugh, {Mary I.} and Lisa Couch and Mengli Xiong and Chinault, {A. Craig} and Antonio Baldini and Caskey, {C. Thomas}",
note = "Funding Information: M.W. was supported by grant We 1470-2 of the Deutsche Forschungsgemein-schaft. The work was supported in part by DOE grant DE-FGO5-88ER60692 to C.T.C. and by DAMDI7-94-J-4484 to C.C.L. C.T.C. is an Investigator with the Howard Hughes Medical Institute. C.C.L. is an investigator with the Clayton Foundation for Research. This research is supported in whole and in part by the Clayton Foundation for Research. The authors thank the Informatics Core of the Human Genome Center at Baylor College of Medicine for computation support. The chromosome specific gene library LL0XNC01 'U' used in this work was constructed at the Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore CA 94550 under the auspices of the National Laboratory Gene Library Project sponsored by the U.S. Department of Energy.",
year = "1995",
month = aug,
doi = "10.1093/hmg/4.8.1373",
language = "English (US)",
volume = "4",
pages = "1373--1380",
journal = "Human molecular genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "8",
}