Isolation of extracellular RNA from serum/plasma

Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, Anil K. Sood

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-57
Number of pages15
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Keywords

  • Cell biology
  • Extracellular RNA
  • Extracellular Vesicle
  • Isolation
  • Molecular Biology
  • Nucleic acids
  • Purification and separation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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