Abstract
A method was developed for selectively isolating genes from localized regions of the human genome that are contained in interspecific hybrid cells. Complementary human DNA was prepared from a human-rodent somatic cell hybrid that contained less than 1% human DNA, by using consensus 5′ intron splice sequences as primers. These primers would select immature, unspliced messenger RNA (still retaining species-specific repeat sequences) as templates. Screening a derived complementary DNA library for human repeat sequences resulted in the isolation of human clones at the anticipated frequency with characteristics expected of exons of transcribed human genes-single copy sequences that hybridized to discrete bands on Northern (RNA) blots.
Original language | English (US) |
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Pages (from-to) | 813-815 |
Number of pages | 3 |
Journal | Science |
Volume | 246 |
Issue number | 4931 |
DOIs | |
State | Published - 1989 |
ASJC Scopus subject areas
- General