Iterative in situ click chemistry creates antibody-like protein-capture agents

Heather D. Agnew; Rosemary D. Rohde; Steven W. Millward; Arundhati Nag; Woon Seok Yeo; Jason E. Hein; Suresh M. Pitram; V. Abdul Ahad Tariq; Anessa M. Burns; Russell J. Krom; Valery V. Fokin; K. Barry Sharpless; James R. Heath

doi:10.1002/anie.200900488

Iterative in situ click chemistry creates antibody-like protein-capture agents

Heather D. Agnew, Rosemary D. Rohde, Steven W. Millward, Arundhati Nag, Woon Seok Yeo, Jason E. Hein, Suresh M. Pitram, V. Abdul Ahad Tariq, Anessa M. Burns, Russell J. Krom, Valery V. Fokin, K. Barry Sharpless, James R. Heath

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.

Original language	English (US)
Pages (from-to)	4944-4948
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	27
DOIs	https://doi.org/10.1002/anie.200900488
State	Published - Jun 22 2009
Externally published	Yes

Keywords

Click chemistry
Combinatorial chemistry
Inhibitors
Peptides
Protein-capture agents

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200900488

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Agnew, H. D., Rohde, R. D., Millward, S. W., Nag, A., Yeo, W. S., Hein, J. E., Pitram, S. M., Abdul Ahad Tariq, V., Burns, A. M., Krom, R. J., Fokin, V. V., Barry Sharpless, K., & Heath, J. R. (2009). Iterative in situ click chemistry creates antibody-like protein-capture agents. Angewandte Chemie - International Edition, 48(27), 4944-4948. https://doi.org/10.1002/anie.200900488

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abstract = "Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.",

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AU - Agnew, Heather D.

AU - Rohde, Rosemary D.

AU - Millward, Steven W.

AU - Nag, Arundhati

AU - Yeo, Woon Seok

AU - Hein, Jason E.

AU - Pitram, Suresh M.

AU - Abdul Ahad Tariq, V.

AU - Burns, Anessa M.

AU - Krom, Russell J.

AU - Fokin, Valery V.

AU - Barry Sharpless, K.

AU - Heath, James R.

PY - 2009/6/22

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KW - Combinatorial chemistry

KW - Inhibitors

KW - Peptides

KW - Protein-capture agents

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Iterative in situ click chemistry creates antibody-like protein-capture agents

Abstract

Keywords

ASJC Scopus subject areas

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