Abstract
In this study we elucidated the role of nonactive JNK in regulating p53 stability. The amount of p53-JNK complex was inversely correlated with p53 level. A peptide corresponding to the JNK binding site on p53 efficiently blocked ubiquitination of p53. Similarly, p53 lacking the JNK binding site exhibits a longer half-life than p53(wt). Outcompeting JNK association with p53 increased the level of p53, whereas overexpression of a phosphorylation mutant form of JNK inhibited p53 accumulation. JNK-p53 and Mdm2-p53 complexes were preferentially found in G0/G1 and S/G2M phases of the cell cycle, respectively. Altogether, these data indicate that JNK is an Mdm2-independent regulator of p53 stability in nonstressed cells.
Original language | English (US) |
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Pages (from-to) | 2658-2663 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 12 |
Issue number | 17 |
DOIs | |
State | Published - Sep 1 1998 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Developmental Biology