KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas

Christian A. Kunder; Giovanna Roncador; Ranjana H. Advani; Gabriela Gualco; Carlos E. Bacchi; Jean M. Sabile; Izidore S. Lossos; Kexin Nie; Robert John Tibshirani; Michael R. Green; Ash A. Alizadeh; Yasodha Natkunam

doi:10.1093/AJCP/AQX099

KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas

Christian A. Kunder, Giovanna Roncador, Ranjana H. Advani, Gabriela Gualco, Carlos E. Bacchi, Jean M. Sabile, Izidore S. Lossos, Kexin Nie, Robert John Tibshirani, Michael R. Green, Ash A. Alizadeh, Yasodha Natkunam

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.

Original language	English (US)
Pages (from-to)	465-476
Number of pages	12
Journal	American Journal of Clinical Pathology
Volume	148
Issue number	6
DOIs	https://doi.org/10.1093/AJCP/AQX099
State	Published - Dec 1 2017

Keywords

Hematopathology
Immunohistochemistry
KLHL6
Lymphoma

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/AJCP/AQX099

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@article{122f4fde2cd545c683f68d650f9ab15a,

title = "KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas",

abstract = "Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.",

keywords = "Hematopathology, Immunohistochemistry, KLHL6, Lymphoma",

author = "Kunder, {Christian A.} and Giovanna Roncador and Advani, {Ranjana H.} and Gabriela Gualco and Bacchi, {Carlos E.} and Sabile, {Jean M.} and Lossos, {Izidore S.} and Kexin Nie and Tibshirani, {Robert John} and Green, {Michael R.} and Alizadeh, {Ash A.} and Yasodha Natkunam",

note = "Funding Information: From the 1Department of Pathology, 2Department of Medicine, Division of Oncology, 3Department of Health Research and Policy, and 4Department of Statistics, Stanford University, Stanford, CA; 5Lymphoma Group, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain; 6Consultoria em Pathologia, Botucatu, Brazil; 7Division of Hematology-Oncology, Department of Medicine, Sylvester Comprehensive Cancer Center, and Department of Molecular and Cellular Pharmacology, University of Miami, Miami, FL; 8Department of Lymphoma/Myeloma, University of Texas MD Anderson Cancer Center, Houston. Publisher Copyright: {\textcopyright} American Society for Clinical Pathology, 2017.",

year = "2017",

month = dec,

day = "1",

doi = "10.1093/AJCP/AQX099",

language = "English (US)",

volume = "148",

pages = "465--476",

journal = "American Journal of Clinical Pathology",

issn = "0002-9173",

publisher = "American Society of Clinical Pathologists",

number = "6",

}

TY - JOUR

T1 - KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas

AU - Kunder, Christian A.

AU - Roncador, Giovanna

AU - Advani, Ranjana H.

AU - Gualco, Gabriela

AU - Bacchi, Carlos E.

AU - Sabile, Jean M.

AU - Lossos, Izidore S.

AU - Nie, Kexin

AU - Tibshirani, Robert John

AU - Green, Michael R.

AU - Alizadeh, Ash A.

AU - Natkunam, Yasodha

N1 - Funding Information: From the 1Department of Pathology, 2Department of Medicine, Division of Oncology, 3Department of Health Research and Policy, and 4Department of Statistics, Stanford University, Stanford, CA; 5Lymphoma Group, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain; 6Consultoria em Pathologia, Botucatu, Brazil; 7Division of Hematology-Oncology, Department of Medicine, Sylvester Comprehensive Cancer Center, and Department of Molecular and Cellular Pharmacology, University of Miami, Miami, FL; 8Department of Lymphoma/Myeloma, University of Texas MD Anderson Cancer Center, Houston. Publisher Copyright: © American Society for Clinical Pathology, 2017.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.

AB - Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.

KW - Hematopathology

KW - Immunohistochemistry

KW - KLHL6

KW - Lymphoma

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U2 - 10.1093/AJCP/AQX099

DO - 10.1093/AJCP/AQX099

M3 - Article

C2 - 29140403

AN - SCOPUS:85038129505

SN - 0002-9173

VL - 148

SP - 465

EP - 476

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

IS - 6

ER -

KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas

Abstract

Keywords

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