Abstract
Background: We have developed a method, termed laser-activated nano-thermolysis as a cell elimination technology (LANTCET), for the selective detection and destruction of individual tumor cells by the generation of intracellular photothermal bubbles around cluster's of gold nanoparticles. Method: Bare nanoparticles and their conjugates to C225 tumor specific monoclonal antibodies were applied in vitro to C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat in order to form intracellular clusters of nanoparticles. Results: Single 10 ns laser pulses generated intracellular photothermal microbubbles at a near-infrared and visible wavelengths. The cells with the clusters yielded an almost 100-fold decrease in the laser fluence threshold for bubble generation and cell damage relative to that for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage and optical guidance) were realized as a microsecond sequence and with the one device.
Original language | English (US) |
---|---|
Pages (from-to) | 647-667 |
Number of pages | 21 |
Journal | Nanomedicine |
Volume | 3 |
Issue number | 5 |
DOIs | |
State | Published - Oct 2008 |
Keywords
- Bubble
- Cluster
- EGF
- Gold nanoparticle
- Laser
- Photothermal
- Squamous carcinoma
- Thermolysis
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
- General Materials Science