TY - JOUR
T1 - Large field, high resolution full-field optical coherence tomography
T2 - A pre-clinical study of human breast tissue and cancer assessment
AU - Assayag, Osnath
AU - Antoine, Martine
AU - Sigal-Zafrani, Brigitte
AU - Riben, Michael
AU - Harms, Fabrice
AU - Burcheri, Adriano
AU - Grieve, Kate
AU - Dalimier, Eugénie
AU - De Poly, Bertrand Le Conte
AU - Boccara, Claude
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2014/10
Y1 - 2014/10
N2 - We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist's management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 mm3 resolution and 200 mm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes.75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range: 25-83). Pathologists blind diagnosed normal/benign or malignant tissue based on FF-OCT images alone, diagnosis from histopathology followed for comparison. The contrast in the FF-OCT images is generated by intrinsic tissue scattering properties, meaning that no tissue staining or preparation is required. Major architectural features and tissue structures of benign breast tissue, including adipocytes, fibrous stroma, lobules and ducts were characterized. Subsequently, features resulting from pathological modification were characterized and a diagnosis decision tree was developed. Using FF-OCT images, two breast pathologists were able to distinguish normal/benign tissue from lesional with a sensitivity of 94% and 90%, and specificity of 75% and 79% respectively.
AB - We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist's management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 mm3 resolution and 200 mm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes.75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range: 25-83). Pathologists blind diagnosed normal/benign or malignant tissue based on FF-OCT images alone, diagnosis from histopathology followed for comparison. The contrast in the FF-OCT images is generated by intrinsic tissue scattering properties, meaning that no tissue staining or preparation is required. Major architectural features and tissue structures of benign breast tissue, including adipocytes, fibrous stroma, lobules and ducts were characterized. Subsequently, features resulting from pathological modification were characterized and a diagnosis decision tree was developed. Using FF-OCT images, two breast pathologists were able to distinguish normal/benign tissue from lesional with a sensitivity of 94% and 90%, and specificity of 75% and 79% respectively.
KW - Breast cancer imaging
KW - Optical coherence tomography
KW - Optical imaging
UR - http://www.scopus.com/inward/record.url?scp=84902189152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902189152&partnerID=8YFLogxK
U2 - 10.7785/tcrt.2013.600254
DO - 10.7785/tcrt.2013.600254
M3 - Article
C2 - 24000981
AN - SCOPUS:84902189152
SN - 1533-0346
VL - 13
SP - 455
EP - 468
JO - Technology in Cancer Research and Treatment
JF - Technology in Cancer Research and Treatment
IS - 5
ER -