TY - JOUR
T1 - Large-pore polydimethylacrylamide resin for solid-phase peptide synthesis
T2 - Applications in Fmoc chemistry
AU - Sparrow, James T.
AU - Knieb-Cordonier, Nancy G.
AU - Obeyseskere, Nihal U.
AU - McMurray, John S.
PY - 1996/11
Y1 - 1996/11
N2 - We have synthesized a hydrophilic, crosslinked, aminoalkyl polydimethylacrylamide-beaded support upon which peptides have been assembled using standard Fmoc chemistry in automated batch-wise equipment. The resin was prepared by the free radical-initiated co-polymerization of N,N-dimethylacrylamide, N,N'-bisacrylyl-1,3-diaminopropane and a functional monomer, N-methacrylyl-1,3-diaminopropane hydrochloride. After coupling of N-α-tert-butyloxycarbonyl-glycine (Bocglycine), amino acid analyses gave resin loading capacities of 0.66 mmol/g. The resulting polymer was highly swollen by polar solvents including aqueous buffers and had an exclusion limit of 50 kDa for soluble proteins. This resin was found to be an excellent support for peptide synthesis using Fmoc chemistry. Typical purities of crude peptides were 80%-95%, including sequences that failed on conventional polystyrene resins.
AB - We have synthesized a hydrophilic, crosslinked, aminoalkyl polydimethylacrylamide-beaded support upon which peptides have been assembled using standard Fmoc chemistry in automated batch-wise equipment. The resin was prepared by the free radical-initiated co-polymerization of N,N-dimethylacrylamide, N,N'-bisacrylyl-1,3-diaminopropane and a functional monomer, N-methacrylyl-1,3-diaminopropane hydrochloride. After coupling of N-α-tert-butyloxycarbonyl-glycine (Bocglycine), amino acid analyses gave resin loading capacities of 0.66 mmol/g. The resulting polymer was highly swollen by polar solvents including aqueous buffers and had an exclusion limit of 50 kDa for soluble proteins. This resin was found to be an excellent support for peptide synthesis using Fmoc chemistry. Typical purities of crude peptides were 80%-95%, including sequences that failed on conventional polystyrene resins.
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M3 - Article
C2 - 9048423
AN - SCOPUS:0030291922
SN - 1040-5704
VL - 9
SP - 297
EP - 304
JO - Peptide research
JF - Peptide research
IS - 6
ER -