Abstract
Dehydrin like transcripts (drought inducible mRNA) accumulated in drought stressed shoots and roots of Lathyrus sativus. ABA also induced the expression of dehydrin in unstressed seedlings but to a lower extent. A cDNA expression library was prepared in lambda ZAP vector from mRNA of drought stressed shoots of Lathyrus sativus cv P-24 and screened with pea dehydrin cDNA probe. One positive clone (∼ 500 bp) was purified and characterised. The partial nucleotide sequence of the clone Lsd, contained a possible open reading frame which would encode a polypeptide with region of significant amino acid sequence similarity to dehydrin from other plant species. The genomic counterpart of Lsd cDNA was found to be present within a fragment of ∼ 3.4 and ∼ 3.2 kb in parent as well as in the somaclones of Lathyrus sativus, by using different restriction enzymes.
Original language | English (US) |
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Pages (from-to) | 97-101 |
Number of pages | 5 |
Journal | Journal of Plant Biochemistry and Biotechnology |
Volume | 5 |
Issue number | 2 |
DOIs | |
State | Published - Jul 1996 |
Keywords
- ABA
- dehydrin
- drought induced
- gene expression
- Lathyrus sativus
- somaclones
ASJC Scopus subject areas
- Biotechnology
- Plant Science
- Agronomy and Crop Science
- General Biochemistry, Genetics and Molecular Biology
- Biochemistry