LC–MS/MS determination of D-mannose in human serum as a potential cancer biomarker

Lyndsey White, Jing Ma, Su Liang, Beatriz Sanchez-Espiridion, Dong Liang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC–MS/MS method development and validation of D-mannose in human serum. Surrogate blank serum, coupled with stable isotope D-mannose-13C6, as internal standard, was used for generating standard curves ranging from 1 to 50 μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0.5 mL/min at 80 °C. Mass detection was performed under negative ionization electrospray. Inter- and intra-day accuracy and precision were <2%. The extraction recovery and matrix effect were 104.1%–105.5% and 97.0%–100.0%, respectively. This method was successfully applied for the quantification of D-mannose in the serum samples of 320 esophageal cancer patients and 323 healthy volunteers. We report a simple, specific and reproducible LC–MS/MS method for the quantification of D-mannose in human serum as a potential cancer biomarker.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume137
DOIs
StatePublished - Apr 15 2017

Keywords

  • Biomarker
  • Esophageal cancer
  • LC–MS/MS
  • Mannose
  • Serum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'LC–MS/MS determination of D-mannose in human serum as a potential cancer biomarker'. Together they form a unique fingerprint.

Cite this