TY - JOUR
T1 - LC–MS/MS determination of D-mannose in human serum as a potential cancer biomarker
AU - White, Lyndsey
AU - Ma, Jing
AU - Liang, Su
AU - Sanchez-Espiridion, Beatriz
AU - Liang, Dong
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/4/15
Y1 - 2017/4/15
N2 - Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC–MS/MS method development and validation of D-mannose in human serum. Surrogate blank serum, coupled with stable isotope D-mannose-13C6, as internal standard, was used for generating standard curves ranging from 1 to 50 μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0.5 mL/min at 80 °C. Mass detection was performed under negative ionization electrospray. Inter- and intra-day accuracy and precision were <2%. The extraction recovery and matrix effect were 104.1%–105.5% and 97.0%–100.0%, respectively. This method was successfully applied for the quantification of D-mannose in the serum samples of 320 esophageal cancer patients and 323 healthy volunteers. We report a simple, specific and reproducible LC–MS/MS method for the quantification of D-mannose in human serum as a potential cancer biomarker.
AB - Several metabolites in human serum have been identified as potential cancer biomarkers for early detection. This study focuses on the LC–MS/MS method development and validation of D-mannose in human serum. Surrogate blank serum, coupled with stable isotope D-mannose-13C6, as internal standard, was used for generating standard curves ranging from 1 to 50 μg/mL. Separation was achieved by an Agilent 1200 series HPLC equipped with a SUPELCOGELTM Pb, 6% Crosslinked column with HPLC water as a mobile phase at flow rate of 0.5 mL/min at 80 °C. Mass detection was performed under negative ionization electrospray. Inter- and intra-day accuracy and precision were <2%. The extraction recovery and matrix effect were 104.1%–105.5% and 97.0%–100.0%, respectively. This method was successfully applied for the quantification of D-mannose in the serum samples of 320 esophageal cancer patients and 323 healthy volunteers. We report a simple, specific and reproducible LC–MS/MS method for the quantification of D-mannose in human serum as a potential cancer biomarker.
KW - Biomarker
KW - Esophageal cancer
KW - LC–MS/MS
KW - Mannose
KW - Serum
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U2 - 10.1016/j.jpba.2016.12.017
DO - 10.1016/j.jpba.2016.12.017
M3 - Article
C2 - 28092855
AN - SCOPUS:85009211720
SN - 0731-7085
VL - 137
SP - 54
EP - 59
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -