Linearizer gene circuits with negative feedback regulation.

Dmitry Nevozhay; Rhys M. Adams; Gábor Balázsi

doi:10.1007/978-1-61779-086-7_5

Linearizer gene circuits with negative feedback regulation.

Dmitry Nevozhay, Rhys M. Adams, Gábor Balázsi

Imaging Physics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.

Original language	English (US)
Pages (from-to)	81-100
Number of pages	20
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	734
DOIs	https://doi.org/10.1007/978-1-61779-086-7_5
State	Published - 2011

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-61779-086-7_5

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abstract = "Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.",

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AB - Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.

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Linearizer gene circuits with negative feedback regulation.

Abstract

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