Abstract
Liposome-encapsulated muramyl tripeptide phosphatidylethanolamine (L-MTP-PE) is a novel immune modulator that is now under investigation against metastatic melanoma and osteosarcoma. We have already reported that L-MTP-PE induced monocyte-mediated tumoricidal activity and up-regulation of the tumor necrosis factor and interleukin-1 (IL-1) in vivo and in vitro. We now demonstrate that L-MTP-PE also induces monocyte chemotactic and activating factor (MCAF) mRNA expression at both the transcriptional and post-transcriptional levels. Monocyte chemotactic activity was also present in the supernatants of L-MTP-PE-stimulated cells. In monocytes, the increased expression of MCAF was induced rapidly (by 2 h) but was short-lived. By 4 h, MCAF mRNA had decreased to background level. We found no change in MCAF mRNA levels in lymphocytes exposed to L-MTP-PE. We therefore conclude that L-MTP-PE selectively up-regulates MCAF expression in monocytes and that MCAF may play a role in the tumoricidal and immune-stimulating activity of L-MTP-PE.
Original language | English (US) |
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Pages (from-to) | 16-22 |
Number of pages | 7 |
Journal | Cancer Immunology Immunotherapy |
Volume | 38 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1994 |
Keywords
- Liposome-encapsulated muramyl tripeptide
- MCAF
- mRNA, monocyte
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Oncology
- Cancer Research