TY - JOUR
T1 - Liquid chromatogrphic determination of daunorubicin and daunorubicinol in plasma from leukemic patients
AU - Eksborg, Staffan
AU - Ehrsson, Hans
AU - Andersson, Börje
AU - Beran, Miloslav
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1978/6/1
Y1 - 1978/6/1
N2 - A method is given for the determination of daunorubicin and its main metabolite, daunorubicinol, in plasma from leukemic patients after administration of daunorubicin as the free drug or as a complex with DNA. Daunorubicin and daunorubicinol are extracted from 2 ml of plasma (pH 8.1) using mixture of chloroform and 1-heptanol (9:1). After re-extraction into phosphoric acid (0.1 M), the separation is performed as reversed phase liquid chromatography on a LiChrosorb RP-2 (5 μm) column with a mobile phase of acetonitrile-water, acidified with phosphoric acid. The precision, by quantitation with a photometric detector, was better than 2% within the range 20 ng/ml to 200 ng/ml. Some determinations of plasma levels of daunorubicin and daunorubicinol are presented.
AB - A method is given for the determination of daunorubicin and its main metabolite, daunorubicinol, in plasma from leukemic patients after administration of daunorubicin as the free drug or as a complex with DNA. Daunorubicin and daunorubicinol are extracted from 2 ml of plasma (pH 8.1) using mixture of chloroform and 1-heptanol (9:1). After re-extraction into phosphoric acid (0.1 M), the separation is performed as reversed phase liquid chromatography on a LiChrosorb RP-2 (5 μm) column with a mobile phase of acetonitrile-water, acidified with phosphoric acid. The precision, by quantitation with a photometric detector, was better than 2% within the range 20 ng/ml to 200 ng/ml. Some determinations of plasma levels of daunorubicin and daunorubicinol are presented.
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U2 - 10.1016/S0021-9673(00)89874-X
DO - 10.1016/S0021-9673(00)89874-X
M3 - Article
C2 - 659557
AN - SCOPUS:0017815554
SN - 0021-9673
VL - 153
SP - 211
EP - 218
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
ER -