TY - JOUR
T1 - Loss of retinoblastoma gene function and heterozygosity at the RB locus in renal cortical neoplasms
AU - Lai, Syeling
AU - Benedict, William F.
AU - Silver, Susan A.
AU - El-Naggar, Adel K.
N1 - Funding Information:
From the Departments of Pathology and Hematology, the University of Texas, M.D. Anderson Cancer Center, Houston, TX; and Gynecologic and Breast Pathology, Armed Forces Institute of Pathology, Washington, DC. Accepted for publication October 14, 1997. Funded in part by grant CA-54672 from the National Cancer Institute. Address correspondence and reprint requests to Adel I~ E1-Naggar. MD, Department of Pathology, the University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd, Box 85, Houston, TX 77030. Copyright © 1997 by W.B. Saunders Company 0046-8177/97/2806-001655.00/0
PY - 1997
Y1 - 1997
N2 - Alteration of the retinoblastoma (RB) gene, located on chromosome 13q14, has been implicated in the pathogenesis and biological behavior of several human cancers. We investigated the RB gene status by Western blotting and immunohistochemical analysis, as well as loss of heterozygosity (LOH) at the RB locus in 21 primary human renal neoplasms (including 3 oncocytomas). In only 1 of 21 tumors was there a discrepancy between Western blot and immunochemical staining. Overall, LOH was noted in 6 of 12 informative cases. However, only one of the tumors with LOH at the RB locus had loss of RB protein expression by both Western blot and immunohistochemical analysis. Loss of RB function was found in 4 of 18 carcinomas and in none of 3 oncocytomas as determined by absent RB nuclear staining in tumor cells. LOH at chromosome 13q14 was more noted in high-grade, DNA aneuploid, high-stage tumors and in patients with poor outcome. These results imply that (1) there is likely another tumor-suppressor gene on chromosome 13 involved in renal carcinogenesis, (2) LOH at chromosome 13q loci may be associated with aggressive behavior, and (3) the loss of RB function may have a role in a subset of renal carcinomas.
AB - Alteration of the retinoblastoma (RB) gene, located on chromosome 13q14, has been implicated in the pathogenesis and biological behavior of several human cancers. We investigated the RB gene status by Western blotting and immunohistochemical analysis, as well as loss of heterozygosity (LOH) at the RB locus in 21 primary human renal neoplasms (including 3 oncocytomas). In only 1 of 21 tumors was there a discrepancy between Western blot and immunochemical staining. Overall, LOH was noted in 6 of 12 informative cases. However, only one of the tumors with LOH at the RB locus had loss of RB protein expression by both Western blot and immunohistochemical analysis. Loss of RB function was found in 4 of 18 carcinomas and in none of 3 oncocytomas as determined by absent RB nuclear staining in tumor cells. LOH at chromosome 13q14 was more noted in high-grade, DNA aneuploid, high-stage tumors and in patients with poor outcome. These results imply that (1) there is likely another tumor-suppressor gene on chromosome 13 involved in renal carcinogenesis, (2) LOH at chromosome 13q loci may be associated with aggressive behavior, and (3) the loss of RB function may have a role in a subset of renal carcinomas.
KW - Immunohistochemistry
KW - Loss of heterozygosity
KW - Renal cell carcinoma
KW - Restriction fragment length polymorphism
KW - Retinoblastoma
UR - http://www.scopus.com/inward/record.url?scp=0030911222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030911222&partnerID=8YFLogxK
U2 - 10.1016/S0046-8177(97)90178-7
DO - 10.1016/S0046-8177(97)90178-7
M3 - Article
C2 - 9191003
AN - SCOPUS:0030911222
SN - 0046-8177
VL - 28
SP - 693
EP - 697
JO - Human Pathology
JF - Human Pathology
IS - 6
ER -