Abstract
Antiserum specific for thymine-containing dimers was used to assay DNA isolated from ultraviolet-irradiated cells following different repair periods. A 50% loss in antibody-binding sites was evident 1 h post-irradiation, and within 4 h 80` of the sites were removed. This result contrasts with data obtained with dimer-specific T4 endonuclease V and does not appear to be due to masking of the dimers by repair enzymes. T4 endonuclease V treatment of ultraviolet-irradiated DNA at 0°C resulted in conversion of the thymine dimers to apyrimidinic sites. This did not result in loss of antigenicity in either PM2 or CHO cell DNA. Likewise, treatment of ultraviolet-irradiated CHO cell DNA with T4 endonuclease at 37°C did not change its antigenicity. These results suggest that aglycosylation of the dimers is not responsible for their inability to bind dimer-specific antibody 2-4 h post-irradiation. The possibility that T4 endonuclease V and the antiserum have different specificities for different dimers is discussed.
Original language | English (US) |
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Pages (from-to) | 270-277 |
Number of pages | 8 |
Journal | BBA - Gene Structure and Expression |
Volume | 697 |
Issue number | 3 |
DOIs | |
State | Published - Jun 30 1982 |
Keywords
- DNA repair
- Radioimmunoassay
- T4 endonuclease V
- Thymine dimer
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics