TY - JOUR
T1 - Low frequency of alterations of the α (PPP2R1A) and β (PPP2R1B) isoforms of the subunit A of the serine-threonine phosphatase 2A in human neoplasms
AU - Calin, George A.
AU - Di Iasio, Maria Grazia
AU - Caprini, Elisabetta
AU - Vorechovsky, Igor
AU - Natali, Pier Giorgio
AU - Sozzi, Gabriella
AU - Croce, Carlo M.
AU - Barbanti-Brodano, Giuseppe
AU - Russo, Giandomenico
AU - Negrini, Massimo
N1 - Funding Information:
We thank Augusto Bevilacqua and Pietro Zucchini for the excellent technical assistance. MG di Iasio is supported by a fellowship from Fondazione Italiana per la Ricerca sul Cancro (FIRC). This work was supported by the European Commission Project QLRT-1999-00786 and by the Asso-ciazione Italiana per la Ricerca sul Cancro (AIRC).
PY - 2000/2/24
Y1 - 2000/2/24
N2 - The phosphatase 2A (PP2A) is one of the major cellular serine-threonine phosphatases. It was recently shown that the gene encoding for the β isoform of its subunit A, PPP2R1B, is altered in human lung and colorectal carcinomas, suggesting a role in human tumorigenesis. Here, we report the detection of mutations in breast, lung carcinomas and melanomas in the genes of both a (PPP2R1A) and β isoforms. Mutations affecting PPP2R1B were found in four breast carcinomas, while mutations in PPP2R1A were found in carcinomas of the breast and of the lung and in one melanoma. Most of the mutations affecting PPP2R1B were exons deletions, suggesting abnormal splicing. These splicing abnormalities were detected in tumor samples in the absence of the normal splicing product, and were not found in several normal controls. In one case, a homozygous deletion present in tumor DNA, and not in the matched normal control was demonstrated. Mutations affecting the PPP2R1A gene were nucleotide substitutions changing highly conserved amino acids and one frame-shift. Although the frequency of alterations is low, the inclusion of both isoforms of subunit A in the genes mutated in human cancer and the addition of breast cancer to the list of neoplasms in which PPP2R1B is altered, strengthen the potential role of PP2A in human tumorogenesis.
AB - The phosphatase 2A (PP2A) is one of the major cellular serine-threonine phosphatases. It was recently shown that the gene encoding for the β isoform of its subunit A, PPP2R1B, is altered in human lung and colorectal carcinomas, suggesting a role in human tumorigenesis. Here, we report the detection of mutations in breast, lung carcinomas and melanomas in the genes of both a (PPP2R1A) and β isoforms. Mutations affecting PPP2R1B were found in four breast carcinomas, while mutations in PPP2R1A were found in carcinomas of the breast and of the lung and in one melanoma. Most of the mutations affecting PPP2R1B were exons deletions, suggesting abnormal splicing. These splicing abnormalities were detected in tumor samples in the absence of the normal splicing product, and were not found in several normal controls. In one case, a homozygous deletion present in tumor DNA, and not in the matched normal control was demonstrated. Mutations affecting the PPP2R1A gene were nucleotide substitutions changing highly conserved amino acids and one frame-shift. Although the frequency of alterations is low, the inclusion of both isoforms of subunit A in the genes mutated in human cancer and the addition of breast cancer to the list of neoplasms in which PPP2R1B is altered, strengthen the potential role of PP2A in human tumorogenesis.
KW - Human neoplasms
KW - Mutations
KW - PPP2R1A
KW - PPP2R1B
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U2 - 10.1038/sj.onc.1203389
DO - 10.1038/sj.onc.1203389
M3 - Article
C2 - 10713707
AN - SCOPUS:0034708257
SN - 0950-9232
VL - 19
SP - 1191
EP - 1195
JO - Oncogene
JF - Oncogene
IS - 9
ER -