Lymphokine activated killer (LAK) cell mediated killing of human glioma: Effect of pretreating glioma with various membrane modifying agents

Steven K. Jacobs; Catherine W. Parham; Bud Holcomb; Barbara Ikejiri; Paul L. Kornblith; Elizabeth A. Grimm

doi:10.1007/BF00162760

Lymphokine activated killer (LAK) cell mediated killing of human glioma: Effect of pretreating glioma with various membrane modifying agents

Steven K. Jacobs, Catherine W. Parham, Bud Holcomb, Barbara Ikejiri, Paul L. Kornblith, Elizabeth A. Grimm

Melanoma Medical Oncology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The killing of human glioma by lymphokine activated killer (LAK) cells was studied. LAK cells generated by culturing recombinant interleukin-2 (ILr2) with human peripheral blood lymphocytes (PBL) obtained from normal volunteers markedly lysed allogeneic glioma grown in tissue culture. Susceptibility of glioma to lysis by LAK cells was abrogated by pretreating the glioma cells with trypsin or chymotrypsin, but was unaffected by pretreatment with hydrocortisone, neuraminidase, glycosidases or sodium periodate. These results suggest that the cell surface determinant on human glioma cells responsible for its tumor selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.

Original language	English (US)
Pages (from-to)	5-10
Number of pages	6
Journal	Journal of neuro-oncology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/BF00162760
State	Published - Mar 1987

Keywords

LAK cells
cytotoxic lymphocytes
glioma
interleukin-2
trypsin

ASJC Scopus subject areas

Oncology
Neurology
Clinical Neurology
Cancer Research

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10.1007/BF00162760

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title = "Lymphokine activated killer (LAK) cell mediated killing of human glioma: Effect of pretreating glioma with various membrane modifying agents",

abstract = "The killing of human glioma by lymphokine activated killer (LAK) cells was studied. LAK cells generated by culturing recombinant interleukin-2 (ILr2) with human peripheral blood lymphocytes (PBL) obtained from normal volunteers markedly lysed allogeneic glioma grown in tissue culture. Susceptibility of glioma to lysis by LAK cells was abrogated by pretreating the glioma cells with trypsin or chymotrypsin, but was unaffected by pretreatment with hydrocortisone, neuraminidase, glycosidases or sodium periodate. These results suggest that the cell surface determinant on human glioma cells responsible for its tumor selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.",

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T2 - Effect of pretreating glioma with various membrane modifying agents

AU - Jacobs, Steven K.

AU - Parham, Catherine W.

AU - Holcomb, Bud

AU - Ikejiri, Barbara

AU - Kornblith, Paul L.

AU - Grimm, Elizabeth A.

PY - 1987/3

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AB - The killing of human glioma by lymphokine activated killer (LAK) cells was studied. LAK cells generated by culturing recombinant interleukin-2 (ILr2) with human peripheral blood lymphocytes (PBL) obtained from normal volunteers markedly lysed allogeneic glioma grown in tissue culture. Susceptibility of glioma to lysis by LAK cells was abrogated by pretreating the glioma cells with trypsin or chymotrypsin, but was unaffected by pretreatment with hydrocortisone, neuraminidase, glycosidases or sodium periodate. These results suggest that the cell surface determinant on human glioma cells responsible for its tumor selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.

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KW - trypsin

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Lymphokine activated killer (LAK) cell mediated killing of human glioma: Effect of pretreating glioma with various membrane modifying agents

Abstract

Keywords

ASJC Scopus subject areas

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