Lymphokine activateed killer (LAK) cell-mediated lysis of murine glioma: trypsinchymotrypsin-sensitive glioma protein is responsible for tumor-selective recognition by LAK cells

Steven K. Jacobs; Gilbert Melin; Bud Holcomb; Catherine W. Parham; Paul L. Kornblith; Elizabeth A. Grimm

doi:10.1016/0006-8993(86)91150-9

Lymphokine activateed killer (LAK) cell-mediated lysis of murine glioma: trypsinchymotrypsin-sensitive glioma protein is responsible for tumor-selective recognition by LAK cells

Steven K. Jacobs, Gilbert Melin, Bud Holcomb, Catherine W. Parham, Paul L. Kornblith, Elizabeth A. Grimm

Melanoma Medical Oncology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The killing of Fischer rat 9L glioma in vitro by lymphokine-activated killer (LAK) cells was studied. LAK cells generated by culturing Fischer spleen cells with recombinant interleukin 2 markedly lysed glioma cells but did not kill syngeneic normal brain tissue in a chromium release microcytotoxicity assay. Susceptibility of glioma to lysis by LAK cells was markedly diminished by pretreating the glioma cells with trypsin or chymotrypsin but was unaffected by pretreatment with neuraminidase, glycosidases, or sodium periodate. These results suggest that LAK cell killing of glioma is probably tumor-selective and that a crucial cell surface determinant on glioma cells responsible for its tumot-selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.

Original language	English (US)
Pages (from-to)	386-389
Number of pages	4
Journal	Brain Research
Volume	372
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(86)91150-9
State	Published - May 7 1986

Keywords

LAK cell
cytotoxic lymphocyte
glioma
interleukin-2
trypsin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(86)91150-9

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title = "Lymphokine activateed killer (LAK) cell-mediated lysis of murine glioma: trypsinchymotrypsin-sensitive glioma protein is responsible for tumor-selective recognition by LAK cells",

abstract = "The killing of Fischer rat 9L glioma in vitro by lymphokine-activated killer (LAK) cells was studied. LAK cells generated by culturing Fischer spleen cells with recombinant interleukin 2 markedly lysed glioma cells but did not kill syngeneic normal brain tissue in a chromium release microcytotoxicity assay. Susceptibility of glioma to lysis by LAK cells was markedly diminished by pretreating the glioma cells with trypsin or chymotrypsin but was unaffected by pretreatment with neuraminidase, glycosidases, or sodium periodate. These results suggest that LAK cell killing of glioma is probably tumor-selective and that a crucial cell surface determinant on glioma cells responsible for its tumot-selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.",

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author = "Jacobs, {Steven K.} and Gilbert Melin and Bud Holcomb and Parham, {Catherine W.} and Kornblith, {Paul L.} and Grimm, {Elizabeth A.}",

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T1 - Lymphokine activateed killer (LAK) cell-mediated lysis of murine glioma

T2 - trypsinchymotrypsin-sensitive glioma protein is responsible for tumor-selective recognition by LAK cells

AU - Jacobs, Steven K.

AU - Melin, Gilbert

AU - Holcomb, Bud

AU - Parham, Catherine W.

AU - Kornblith, Paul L.

AU - Grimm, Elizabeth A.

PY - 1986/5/7

Y1 - 1986/5/7

N2 - The killing of Fischer rat 9L glioma in vitro by lymphokine-activated killer (LAK) cells was studied. LAK cells generated by culturing Fischer spleen cells with recombinant interleukin 2 markedly lysed glioma cells but did not kill syngeneic normal brain tissue in a chromium release microcytotoxicity assay. Susceptibility of glioma to lysis by LAK cells was markedly diminished by pretreating the glioma cells with trypsin or chymotrypsin but was unaffected by pretreatment with neuraminidase, glycosidases, or sodium periodate. These results suggest that LAK cell killing of glioma is probably tumor-selective and that a crucial cell surface determinant on glioma cells responsible for its tumot-selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.

AB - The killing of Fischer rat 9L glioma in vitro by lymphokine-activated killer (LAK) cells was studied. LAK cells generated by culturing Fischer spleen cells with recombinant interleukin 2 markedly lysed glioma cells but did not kill syngeneic normal brain tissue in a chromium release microcytotoxicity assay. Susceptibility of glioma to lysis by LAK cells was markedly diminished by pretreating the glioma cells with trypsin or chymotrypsin but was unaffected by pretreatment with neuraminidase, glycosidases, or sodium periodate. These results suggest that LAK cell killing of glioma is probably tumor-selective and that a crucial cell surface determinant on glioma cells responsible for its tumot-selective lysis by LAK is a protein sensitive to trypsin and chymotrypsin.

KW - LAK cell

KW - cytotoxic lymphocyte

KW - glioma

KW - interleukin-2

KW - trypsin

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Lymphokine activateed killer (LAK) cell-mediated lysis of murine glioma: trypsinchymotrypsin-sensitive glioma protein is responsible for tumor-selective recognition by LAK cells

Abstract

Keywords

ASJC Scopus subject areas

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