TY - JOUR
T1 - Macrophage infiltration into experimental brain metastases
T2 - Occurrence through an intact blood-brain barrier
AU - Schackert, Gabriele
AU - Simmons, Rex D.
AU - Buzbee, Thomas M.
AU - Hume, David A.
AU - Fidler, Isaiah J.
N1 - Funding Information:
Supported in part by Public Health Service grants R35-CA-42107 and CA-16672 from the National Cancer Institute, National Institutes of Health, Department of Health and Human Services; and by funds from the Meadows Foundation. G. Schackert is die recipient of a scholarship from the Dr. Mildred Scheel Foundation for Cancer Research, Bonn, Federal Republic of Germany. 'Department of Cell Biology, University of Texas-M. D. Anderson Cancer Center, Houston, TX. 'Department of Pathology, School of Medicine, University of Texas Healdi Science Center at Houston, Houston, TX. *Correspondence to: Dr. Isaiah J. Fidler, Department of Cell Biology (HMB 173), University of Texas-M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030.
PY - 1988/9/7
Y1 - 1988/9/7
N2 - The purpose of this study was to examine the nature of the bllod-brain barrier in experimental brain metastases. Syngeneic fibrosarcoma or melanoma cells were injected into the internal carotid arteries of mice. Several weeks later, once the experimental brain metastases were established, the mice were given injections iv of sodium fluorescein.The capillaries within the metastatic foci were enlarged and irregular, but there was no leakage of sodium fluorescein, showing that the blood-brain barrier was intact. The neoplastic lesions were infiltrated by mononuclear phagocytes, which were identified by immunohistochemical localization of the macrophage-specific antigen F4/80, class II major histocom-patibility complex (MHC) antigens, and the macrophage product interleukin-1(IL-1). The metastatic foci contained numerous stellate macrophages that expressed F4/80 and MHC class II antigens, but little IL-1. Round, Monocyte-like F4/80 and MHC class II-positive cells were also observed within the tumor lesions and adhering to walls of the tumor microvasculature. Mice with fibrosarcoma brain metastases also had edematous lesions at sites remote from the metastatic foci that contained numerous astrocytes expressing class II MHC but not F4/80 antigens. In conclusion, the blood-brain barrier is intact within experimental brain metastases, yet macrophages of blood monocyte origin can infiltrage the lesions. [J Natl Cancer Inst 1988;80:1027-1034].
AB - The purpose of this study was to examine the nature of the bllod-brain barrier in experimental brain metastases. Syngeneic fibrosarcoma or melanoma cells were injected into the internal carotid arteries of mice. Several weeks later, once the experimental brain metastases were established, the mice were given injections iv of sodium fluorescein.The capillaries within the metastatic foci were enlarged and irregular, but there was no leakage of sodium fluorescein, showing that the blood-brain barrier was intact. The neoplastic lesions were infiltrated by mononuclear phagocytes, which were identified by immunohistochemical localization of the macrophage-specific antigen F4/80, class II major histocom-patibility complex (MHC) antigens, and the macrophage product interleukin-1(IL-1). The metastatic foci contained numerous stellate macrophages that expressed F4/80 and MHC class II antigens, but little IL-1. Round, Monocyte-like F4/80 and MHC class II-positive cells were also observed within the tumor lesions and adhering to walls of the tumor microvasculature. Mice with fibrosarcoma brain metastases also had edematous lesions at sites remote from the metastatic foci that contained numerous astrocytes expressing class II MHC but not F4/80 antigens. In conclusion, the blood-brain barrier is intact within experimental brain metastases, yet macrophages of blood monocyte origin can infiltrage the lesions. [J Natl Cancer Inst 1988;80:1027-1034].
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U2 - 10.1093/jnci/80.13.1027
DO - 10.1093/jnci/80.13.1027
M3 - Article
C2 - 3261801
AN - SCOPUS:0023684344
SN - 0027-8874
VL - 80
SP - 1027
EP - 1034
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 13
ER -