Abstract
Mesenchymal precursor cells found in the blood (BMPCs) of normal persons adhere to plastic and glass and proliferate logarithmically in DMEM-20% fetal calf serum (FCS) without growth factors. They form cells with fibroblast-like and stromal morphology, which is not affected by eliminating CD34, CD3, or CD14 cells. Osteogenic supplements (dexamethasone, ascorbic acid, and β-glycerophosphate) added to the culture inhibited fibroblast formation, and BMPCs assumed the cuboidal shape of osteoblasts. After 5 days in supplemented medium, the elutriated cells displayed alkaline phosphatase. (AP), and the addition of bone morphogenetic protein (BMP)2 (1 ng) doubled AP production (P<0.04). Two weeks later, 30% of the cells were very large and reacted with anti-osteocalcin antibody. The same cultures also contained sudanophlic adipocytes and multinucleated giant cells that stained for tartrate-resistant acid phosphatase (TRAP) and vitronectin receptors. Cultured BMPCs immunostain with antibodies to vimentin, type I collagen, and BMP receptors, heterodimeric structures expressed on mesenchymal lineage cells. In addition, BMPCs stain with anti-CD105 (endoglin), a putative marker for bone-marrow mesenchymal stem cells (MSCs).
Original language | English (US) |
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Pages (from-to) | 477-488 |
Number of pages | 12 |
Journal | Arthritis Research |
Volume | 2 |
Issue number | 6 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Adipocytes
- Bone marrow progenitor cells
- Bone morphogenetic protein receptors
- CD34
- Mesenchymal precursor cells
- Osteoblasts
- Osteoclasts
- Rheumatoid arthritis
- SDF-1
ASJC Scopus subject areas
- Rheumatology