Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

Sanford J. Silverman; Allegra A. Petti; Nikolai Slavov; Lance Parsons; Ryan Briehof; Stephan Y. Thiberge; Daniel Zenklusen; Saumil J. Gandhi; Daniel R. Larson; Robert H. Singer; David Botstein

doi:10.1073/pnas.1002422107

Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

Sanford J. Silverman, Allegra A. Petti, Nikolai Slavov, Lance Parsons, Ryan Briehof, Stephan Y. Thiberge, Daniel Zenklusen, Saumil J. Gandhi, Daniel R. Larson, Robert H. Singer, David Botstein

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAsfrom pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.

Original language	English (US)
Pages (from-to)	6946-6951
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	107
Issue number	15
DOIs	https://doi.org/10.1073/pnas.1002422107
State	Published - Apr 13 2010
Externally published	Yes

Keywords

Chemostat
Fluorescence in situ hybridization
mRNA
Single cells

ASJC Scopus subject areas

General

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10.1073/pnas.1002422107

Cite this

Silverman, S. J., Petti, A. A., Slavov, N., Parsons, L., Briehof, R., Thiberge, S. Y., Zenklusen, D., Gandhi, S. J., Larson, D. R., Singer, R. H., & Botstein, D. (2010). Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate. Proceedings of the National Academy of Sciences of the United States of America, 107(15), 6946-6951. https://doi.org/10.1073/pnas.1002422107

Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate. / Silverman, Sanford J.; Petti, Allegra A.; Slavov, Nikolai et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 15, 13.04.2010, p. 6946-6951.

Research output: Contribution to journal › Article › peer-review

Silverman, SJ, Petti, AA, Slavov, N, Parsons, L, Briehof, R, Thiberge, SY, Zenklusen, D, Gandhi, SJ, Larson, DR, Singer, RH & Botstein, D 2010, 'Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 15, pp. 6946-6951. https://doi.org/10.1073/pnas.1002422107

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abstract = "Oscillations in patterns of expression of a large fraction of yeast genes are associated with the {"}metabolic cycle,{"} usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAsfrom pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.",

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AU - Slavov, Nikolai

AU - Parsons, Lance

AU - Briehof, Ryan

AU - Thiberge, Stephan Y.

AU - Zenklusen, Daniel

AU - Gandhi, Saumil J.

AU - Larson, Daniel R.

AU - Singer, Robert H.

AU - Botstein, David

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Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

Abstract

Keywords

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