Abstract
Background and Aims: Galectin 3 is a β-galactoside-binding protein whose expression has been correlated with advanced tumor stage in the colon, but direct evidence for a role in metastasis is lacking. The current study was designed to more directly establish the role of galectin 3 in colon cancer metastasis. Methods: Galectin 3 levels were manipulated in human colon cancer cells using eukaryotic expression constructs designed to express the complete galectin 3 complementary DNA in either the sense or antisense orientation. Liver colonization was assessed in athymic mice after splenic- portal inoculation or after spontaneous metastasis during cecal growth. Results: Introduction of galectin 3 antisense into metastatic colon cancer cells (LSLiM6, HM7) resulted in a significant reduction in galectin 3- specific messenger RNA and total and cell surface galectin 3 protein. Conversely, stable integration of galectin 3 in the sense orientation resulted in an increase in cellular and cell surface galectin 3 in cells of low metastatic potential (LS174T). Reduction in galectin 3 levels was associated with a marked decrease in liver colonization and spontaneous metastasis by LSLiM6 and HM7 cells, whereas upregulation of galectin 3 resulted in increased metastasis by LS174T cells. Conclusions: This study provides direct evidence that galectin 3 plays an important role in colon cancer metastasis.
Original language | English (US) |
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Pages (from-to) | 287-296 |
Number of pages | 10 |
Journal | Gastroenterology |
Volume | 115 |
Issue number | 2 |
DOIs | |
State | Published - 1998 |
ASJC Scopus subject areas
- Hepatology
- Gastroenterology