TY - JOUR
T1 - Methods of detection of circulating melanoma cells
T2 - A comparative overview
AU - Nezos, Andrianos
AU - Msaouel, Pavlos
AU - Pissimissis, Nikolaos
AU - Lembessis, Peter
AU - Sourla, Antigone
AU - Armakolas, Athanasios
AU - Gogas, Helen
AU - Stratigos, Alexandros J.
AU - Katsambas, Andreas D.
AU - Koutsilieris, Michael
PY - 2011/6
Y1 - 2011/6
N2 - Disease dissemination is the major cause of melanoma-related death. A crucial step in the metastatic process is the intravascular invasion and circulation of melanoma cells in the bloodstream with subsequent development of distant micrometastases that is initially clinically undetectable and will eventually progress into clinically apparent metastasis. Therefore, the use of molecular methods to detect circulating melanoma cells may be of value in risk stratification and clinical management of such patients. Herein, we review the currently applied techniques for the detection, isolation, enrichment and further characterization of circulating melanoma cells from peripheral blood samples in melanoma patients. Furthermore, we provide a brief overview of the various molecular markers currently being evaluated as prognostic indicators of melanoma progression.
AB - Disease dissemination is the major cause of melanoma-related death. A crucial step in the metastatic process is the intravascular invasion and circulation of melanoma cells in the bloodstream with subsequent development of distant micrometastases that is initially clinically undetectable and will eventually progress into clinically apparent metastasis. Therefore, the use of molecular methods to detect circulating melanoma cells may be of value in risk stratification and clinical management of such patients. Herein, we review the currently applied techniques for the detection, isolation, enrichment and further characterization of circulating melanoma cells from peripheral blood samples in melanoma patients. Furthermore, we provide a brief overview of the various molecular markers currently being evaluated as prognostic indicators of melanoma progression.
KW - Circulating melanoma cells
KW - Molecular markers
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U2 - 10.1016/j.ctrv.2010.10.002
DO - 10.1016/j.ctrv.2010.10.002
M3 - Review article
C2 - 21106295
AN - SCOPUS:79953099964
SN - 0305-7372
VL - 37
SP - 284
EP - 290
JO - Cancer treatment reviews
JF - Cancer treatment reviews
IS - 4
ER -